Cucurbit Powdery Mildew Race Identification by Triplet-Septet and Disease Progress Estimation

Rabelo, Hudson de Oliveira; Braz, Leila Trevisan; McCreight, James Donald

doi:10.1590/0034-737X202370010011

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Plant Breeding Applied to Agriculture • Rev. Ceres 70 (1) • Jan-Feb 2023 • https://doi.org/10.1590/0034-737X202370010011 copy

Cucurbit Powdery Mildew Race Identification by Triplet-Septet and Disease Progress Estimation¹ 1 This manuscript is a portion of the Doctoral thesis submitted by the first author. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The Triplet-Septet (TS) set of melon cucurbit powdery mildew (CPM) race differentials (CPMRD) was established to provide an international means for objective and uniform identification and designation on CPM races. The Area Under Disease Progress Stairs (AUDPS) method for disease progress estimation was derived from the Area Under Disease Progress Curve (AUDPC) method, and both have been used to evaluate disease progress on other crops. We aimed to identify a melon CPM race on the TS melon CPMRD, and estimate disease progress thereon using AUDPC and AUDPS. Plants were inoculated at the 3 to 4 true leaf stage. Severity of CPM infection was evaluated on the 21 TS melon CPMRD at 15, 22, 32, and 41 days after inoculation (DAI) using a visual scale. The CPM population in the greenhouse was identified as race S based on reactions of a set of 11 commonly used melon CPMRD, and it may also be designated as 127.127.126 on the TS melon CPMRD. AUDPS identified higher levels of disease than AUDPC, and its results agreed with those obtained by the commonly used melon CPMRD conventional race identification methods (current and Triplet-Septet). AUDPS can be used to evaluate the disease progress on CPM.

Keywords
AUDPC; AUDPS; Cucumis melo ; genetic resistance; Podosphaera xanthii

Differential	Mean disease reaction^z z Means followed by the same letter do not differ significantly at P < 0.05 by Tukey HSD test.	Summary reaction^y y R = resistant, S = susceptible;	Triplet-Septet
Differential		Summary reaction^y y R = resistant, S = susceptible;	Group	Weight	Score
Iran H	9.0 a	S	1.1	1	1
Védrantais	9.0 a	S	1.2	2	2
PI 179901	9.0 a	S	1.3	4	4
PI 234607	9.0 a	S	1.4	8	8
AR HBJ	9.0 a	S	1.5	16	16
PMR 45	9.0 a	S	1.6	32	32
PMR 6	8.7 a	S	1.7	64	64
Septet total					127
WMR 29	9.0 a	S	2.1	1	1
Edisto 47	8.7 ab	S	2.2	2	2
PI 414723	9.0 a	S	2.3	4	4
PMR 5	9.0 a	S	2.4	8	8
PI 124112	7.0 b	S	2.5	16	16
MR-1	5.3 c	S	2.6	32	32
PI 124111	7.0 b	S	2.7	64	64
Septet total					127
PI 313970	1.0 d	R	3.1	1	0
Noy Yzre’el	9.0 a	S	3.2	2	2
PI 236355	9.0 a	S	3.3	4	4
Negro	8.3 ab	S	3.4	8	8
Amarillo^x x Reaction based on a single plant, not included in analysis of variance.	9.000	S	3.5	16	16
Nantais Oblong	9.0 a	S	3.6	32	32
Ames 31282	9.0 a	S	3.7	64	64
Septet total					126
F test	40.83^ Significant at P < 0.01 by F test. 0
Mean	8.13
CV (%)	6.16
Alphanumeric race nomenclature: S^w w McCreight and Coffey, 2011.
Triplet-Septet code					127.127.126

Differential	AUDPC^z z Van der Plank, 1963.	AUDPS^y y Simko and Piepho, 2012.	sAUDPC^y y Simko and Piepho, 2012.	sAUDPS^y y Simko and Piepho, 2012.
Iran H	202.50 a^x x Means within columns followed by the same letter do not differ significantly at P < 0.05 by Tukey HSD test.	266.06 a	7.8 a	7.7 a
Védrantais	196.17 ab	259.72 a	07.5 ab	7.5 a
PI 179901	191.33 ab	0252.00 ab	07.4 ab	07.3 ab
PI 234607	170.33 abc	00223.78 abc	006.6 abc	006.5 abc
AR HBJ	196.17 ab	0255.39 ab	07.5 ab	07.4 ab
PMR 45	185.33 ab	0246.00 ab	07.1 ab	07.1 ab
PMR 6	155.00 abc	00201.22 abc	006.0 abc	005.8 abc
WMR 29	198.50 a0	260.61 a	7.6 a	7.5 a
Edisto 47	177.67 abc	00229.67 abc	006.8 abc	006.6 abc
PI 414723	0169.50 abc	00225.83 abc	006.5 abc	006.5 abc
PMR 5	0171.67 abc	00219.33 abc	006.6 abc	006.3 abc
PI 124112	145.50 bc	0190.28 bc	05.6 bc	05.5 bc
MR-1	123.50 c0	165.39 c	4.8 c	4.8 c
PI 124111	128.83 c0	167.83 c	5.0 c	4.8 c
PI 313970	34.50 d	043.17 d	1.3 d	1.2 d
Noy Yzre’el	177.33 abc	0235.11 ab	006.8 abc	06.8 ab
PI 236355	181.07 ab	00231.02 abc	007.0 abc	006.7 abc
Negro	186.83 ab	0243.17 ab	07.2 ab	07.0 ab
Nantais Oblong	192.17 ab	0249.94 ab	07.4 ab	07.2 ab
Ames 31282	182.50 ab	0238.83 ab	07.0 ab	06.9 ab
F test	14.37^ Significant at P < 0.01.	15.89^ Significant at P < 0.01.	14.37^ Significant at P < 0.01.	15.89^ Significant at P < 0.01.
t-test	-30.31^ Significant at P < 0.01.		7.50^ Significant at P < 0.01.
Mean	168.1200	220.05	6.46	6.350
CV (%)	9.98	9.57	9.98	9.570

Disease rating	Disease rating simulated				AUDPC^z z Van der Plank, 1963.	AUDPS^y y Simko and Piepho, 2012.	sAUDPC^y y Simko and Piepho, 2012.	sAUDPS^y y Simko and Piepho, 2012.
Disease rating	15 DAI	22 DAI	32 DAI	41 DAI	AUDPC^z z Van der Plank, 1963.	AUDPS^y y Simko and Piepho, 2012.	sAUDPC^y y Simko and Piepho, 2012.	sAUDPS^y y Simko and Piepho, 2012.
1	1	1	1	1	26.00	34.67	1.00	1.00
2	2	2	2	2	52.00	69.33	2.00	2.00
3	3	3	3	3	78.00	104.00	3.00	3.00
4	4	4	4	4	104.00	138.67	4.00	4.00
5	5	5	5	5	130.00	173.33	5.00	5.00
6	6	6	6	6	156.00	208.00	6.00	6.00
7	7	7	7	7	182.00	242.67	7.00	7.00
8	8	8	8	8	208.00	277.33	8.00	8.00
9	9	9	9	9	234.00	312.00	9.00	9.00

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[1] *Corresponding author: hudsonorabelo@gmail.com