The present study aimed to revalidate the method for determination of ascorbic acid by titration to be applied in the laboratory of Food Chemistry Centre of Agricultural Sciences, Federal University of Espírito Santo. The revalidation process was carried out by evaluating parameters such as linearity, precision (repeatability), accuracy (recovery test) and detection limits, considering the acceptability criteria determined. The level of significance in hypothesis testing was 5%. Analyses included simple linear correlation coefficient (r), coefficient of variation (CV) and regression analysis. The data were analyzed using the software Statistica 7.0. The quantitative method by titration of ascorbic acid was revalidated and evaluated in samples of ascorbic acid and lemon juice. It was found that the same complies with the limits established by regulatory agencies. The methods presented linear, repetitive and high recovery, with a maximum value of 98% and a minimum of 94.82% and a detection limit of 0.025 mg ml-1 in the lemon juice.
titration; intralaboratory validation; food analysis
