The preparation and publication of the technical rules and procedures is a positive investment for all institution that works based on objectives and desires to analyse their results. The authors concerned in contributing to the institutions which intend to implant this service, present in this work the technical rules and procedures connected to the reception and selection of wet - nurses, internal and home collection of the milk preparation and sterilization of the material, the storage and analysis of the milk in laboratories and the destiny of this collected milk. Those technical rules and procedures are adopted by the HUMAN MILK BANK AND INFORMATION CENTER ON MATERNAL MILK, FEEDING OF THE CARMELA DUTRA MATERNITY WARD. They had been worked out based on two years experiment.