SEROLOGICAL AND MOLECULAR SURVEY OF
                        <i>Leptospira</i> spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF
                    HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL

Finger, Mariane Angélica; Barros Filho, Ivan Roque de; Leutenegger, Christian; Estrada, Marko; Ullmann, Leila Sabrina; Langoni, Hélio; Kikuti, Mariana; Dornbush, Peterson Triches; Deconto, Ivan; Biondo, Alexander Welker

doi:10.1590/S0036-46652014000600003

Acessibilidade / Reportar erro

Brasil

Revista do Instituto de Medicina Tropical de São Paulo

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Leptospirosis • Rev. Inst. Med. Trop. São Paulo 56 (6) • Nov-Dec 2014 • https://doi.org/10.1590/S0036-46652014000600003 copy

SEROLOGICAL AND MOLECULAR SURVEY OF Leptospira spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL

Inquérito sorológico e molecular de Leptospira spp. em cavalos carroceiros de área endêmica para leptospirose humana em Curitiba, Sul do Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities.

Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed.

Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03).

Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.

Leptospira spp.; MAT; qPCR; Horses

Sampling	01	02	03
Serovar	Icterohaemorrhagiae	Icterohaemorrhagiae	Icterohaemorrhagiae
Frequency	64% (16/25)	45% (9/20)	50% (11/22)
Serovar	Canicola, Icterohaemorrhagiae	Canicola, Icterohaemorrhagiae	Icterohaemorrhagiae, Castellonis
Frequency	4% (1/25)	5% (1/20)	13.63% (3/22)
Serovar	Bratislava	Bratislava	Icterohaemorrhagiae, Castellonis and Pyrogenes
Frequency	8% (2/25)	5% (1/20)	4.50% (1/22)
Serovar	Canicola	Gryppotyphosa
Frequency	8% (2/25)	5% (1/20)
Serovar	Canicola, Pyrogenes, Hardjo pratijino
Frequency	4% (1/25)
Serovar	Canicola, Pyrogenes
Frequency	4% (1/25)
Serovar	Bratislava, Icterohaemorrhagiae
Frequency	4% (1/25)
Serovar	Pomona
Frequency	4% (1/25)

Animal	Sampling 01	Sampling 02	Sampling 03
01	400 Icterohaemorrhagiae	400 Icterohaemorrhagiae	Not determined
02	200 Icterohaemorrhagiae	400 Icterohaemorrhagiae	400 Icterohaemorrhagiae
03	400 Icterohaemorrhagiae	Not determined	200 Icterohaemorrhagiae, 100 Castellonis
04	Not determined	400 Icterohaemorrhagiae	400 Icterohaemorrhagiae

	November 2010
	Reagent	Non reagent	p
Contact with mud, garbage and sewage
Yes	9 (60%)	3 (42.86%)	0.27
No	6 (40%)	4 (57.14%)
House located less than 20 meters from garbage accumulation
Yes	9 (60%)	6 (85.71%)	0.20
No	6 (40%)	1 (14.29%)
House located less than 10 meters from open sewage
Yes	11 (73.33%)	5 (71.43%)	0.38
No	4 (26.67%)	2 (28.57%)
Presence of rodents near house
Yes	14 (93.33%)	4 (57.14%)	0.07
No	1 (6.67%)	3 (42.86%)
Flooding near house
Yes	8 (53.33%)	0	0.02
No	7 (46.67%)	7 (100%)

Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000 - São Paulo - SP - Brazil, Tel. +55 11 3061-7005 - São Paulo - SP - Brazil
E-mail: revimtsp@usp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Correspondence to: Alexander Welker Biondo. Departamento de Medicina Veterinária, Campus Agrárias, Universidade Federal do Paraná. R. Funcionários, 1540, Juvevê, 80035-050 Curitiba, PR, Brasil. Phone: +55 41 3350 5723; Fax: +55 41 3350 5623. E-mail: abiondo@illinois.edu