Schistosoma mansoni: host cell adhesion to the different stages of the parasite, in vivo

Melo, Alan L.; Pereira, Leógenes H.; Machado, Conceição R. S.

doi:10.1590/S0036-46651992000300004

Abstracts

The peritoneal cavity of laboratory mice was used to study the phenomenon of host cell adhesion to different evolutive stages of the Schistosoma mansoni (cercaria, adult worm, developing and mature eggs, miracidium, young and mature daughter sporocysts). Material recovered from the peritoneal cavity 30 and 180 min after the inoculation of each evolutive form was examined with the help of a stereomicroscope. The free swimming larvae (cercaria and miracidium), and the evolutive forms producing such larvae (mature egg and mature daughter sporocyst) elicited the host cell adhesion phenomenon. In all forms but cercariae the adherent cells remained as so till 180 minutes after inoculation

Schistosoma mansoni; Cell adhesion; Peritoneal cavity

A cavidade peritoneal de camundongos foi utilizada para estudos de adesão celular a diferentes estádios evolutivos do Schistosoma mansoni (cercária, verme adulto, ovos imaturos e maduros, miracídio, esporocisto jovem e esporocisto maduro). O material recuperado da cavidade peritoneal 30 e 180 min após o inóculo, foi examinado com auxílio de estereomicroscópio. As formas livres (cercária e miracídio) e as formas evolutivas que produzem tais larvas (ovo maduro e esporocisto maduro) apresentam células do hospedeiro aderidas à superfície. Em todas as formas, exceto cercária, as células permanecem aderidas pelo menos até 180 min após o inóculo

ORIGINAL ARTICLES

Schistosoma mansoni: host cell adhesion to the different stages of the parasite, in vivo

Schistosoma mansoni: adesão de células do hospedeiro aos diferentes estádios do parasito, in vivo

Alan L. Melo^I; Leógenes H. Pereira^I; Conceição R. S. Machado^II

^IGrupo Interdepartamental de estudos Sobre Esquistossomose/Departamento de Parasitologia - Instituto de Ciências Biológicas da UFMG - Caixa Postal 2486 CEP 30161 Belo Horizonte, Brazil

^IIDepartamento de Morfologia - Instituto de Ciências Biológicas da UFMG - Caixa Postal 2486 CEP 30161 Belo Horizonte, Brazil

SUMMARY

The peritoneal cavity of laboratory mice was used to study the phenomenon of host cell adhesion to different evolutive stages of the Schistosoma mansoni (cercaria, adult worm, developing and mature eggs, miracidium, young and mature daughter sporocysts). Material recovered from the peritoneal cavity 30 and 180 min after the inoculation of each evolutive form was examined with the help of a stereomicroscope. The free swimming larvae (cercaria and miracidium), and the evolutive forms producing such larvae (mature egg and mature daughter sporocyst) elicited the host cell adhesion phenomenon. In all forms but cercariae the adherent cells remained as so till 180 minutes after inoculation.

Key words: Schistosoma mansoni; Cell adhesion; Peritoneal cavity.

RESUMO

A cavidade peritoneal de camundongos foi utilizada para estudos de adesão celular a diferentes estádios evolutivos do Schistosoma mansoni (cercária, verme adulto, ovos imaturos e maduros, miracídio, esporocisto jovem e esporocisto maduro). O material recuperado da cavidade peritoneal 30 e 180 min após o inóculo, foi examinado com auxílio de estereomicroscópio. As formas livres (cercária e miracídio) e as formas evolutivas que produzem tais larvas (ovo maduro e esporocisto maduro) apresentam células do hospedeiro aderidas à superfície. Em todas as formas, exceto cercária, as células permanecem aderidas pelo menos até 180 min após o inóculo.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 26/9/1991.

Aceito para publicação em 11/2/1992.

Supported by FAPEMIG, CNPQ, FINEP (Brazil) and Parasitic Diseases Programme - WHO - Switzerland.

1. ANWAR, A.R.E.; SMITHERS, S.R. & KAY, A.B. - Killing of schistosomula of Schistosoma mansoni coated with antibody and/or complement by human leucocytes in vitro: requirement for complement in preferential killing by eosinophils. J. Immunol., 122: 628-637, 1979.
2. BUTTERWORTH, A.E.; TAYLOR, D.W.; VEITH, M.C.; VADAS, M.A.; DESSEIN, A.; STURROCK, R.F. & WELLS, E. - Studies on the mechanisms of immunity in human schistosomiasis. Immun. Rev., 61: 5-39, 1982.
3. CAPRON, A.; BIGUET, J.; ROSE, F. & VERNES, A. - Les antigčnes de Schistosoma mansoni II. Etude immunoéletrophorétique comparée de divers stades larvaires et des adultes des deux sexes. Aspects immunologiques des relations hôte-parasite de la cercaire et de l'adulte de S. mansoni. Ann. Inst. Pasteur, 109: 798-810, 1965.
4. CHAMONE, M.; MELO, A.L. & PEREIRA, L.H. - Effect of different saline solutions on the cercaricidal activity of normal human sera. Trans. roy. Soc. trop. Med. Hyg., 81: 147, 1987.
5. CHAIA, G. - Técnica para concentraçăo de miracídios. Rev. bras. Malar., 8: 355-357, 1956.
6. CULBERTSON, J.T. - The cercaricidal action of normal serums. J. Parasit., 22: 111-115, 1936.
7. CHIANG, C.P. & CAUFIELD, J.P. - Schistosoma mansoni: Ultrastructural demonstration of a miracidial glycocalyx that cross-reacts with antibodies raised against the cercarial glycocalyx. Exp. Parasit., 67: 63-72, 1988.
8. DALTON, J.P.; LEWIS, S.A.; ARONSTEIN, W.S. & STRAND, M. - Schistosoma mansoni: immunogenic glycoproteins of the cercarial glycocalyx. Exp. Parasit., 63: 215-226, 1987.
9. DIAS DA SILVA, W. & KAZATCHKINE, M.D. - Schistosoma mansoni activation of the alternative pathway of human complement by schistosomula. Exp. Parasit., 50: 278-286, 1980.
10. DUNNE, D.W. & BICKLE, Q.D. - Identification and characterization of a polysaccharide-containing antigen from Schistosoma mansoni eggs which cross-reacts with the surface of schistosomula. Parasitology, 94: 255-268, 1987.
11. DRESDEN, M.H. & DEELDER, A.M. - Schistosoma mansoni: Thiol proteinase properties of adult worm "hemoglobinase". Exp. Parasit., 48: 190-197, 1979.
12. EVELAND, L.K. - Schistosoma mansoni: conversion of cercariae to schistosomula. Exp. Parasit., 32: 261-264, 1972.
13. HARN, D.A.; QUINN, J.J.; CIANCI, C.M. & KO, A.I. - Evidence that protective membrane epitope is involved in early but not late phase immunity in Schistosoma mansoni. J. Immunol., 138: 1571-1580, 1987.
14. KEMP, W.M. - Ultrastructure of the cercarienhullen reaktion of Schistosoma mansoni. J. Parasit., 56: 713-723, 1970.
15. KEMP, W.M.; DAMIAN, R.T. & GREENE, N.D. - Schistosoma mansoni: Immunohistochemical location of CHR in glycocalyx of cercariae. Exp. Parasit., 33: 27-33, 1973.
16. KENT, N.H - Some Biochemical and immunological aspects of host-parasite relationships. Comparative immunochemisty of larval and adult forms of Schistosoma mansoni. Ann. N. Y. Acad. Sci., 113: 100-113, 1963.
17. LANDSPERGER, W.J.; STIREWALT, M.A. & DRESDEN, M.H. - Purification and properties of a proteolytic enzyme from the cercariae of the human trematode parasite Schistosoma mansoni. Biochem. J., 201: 137-144, 1982.
18. MACHADO, A.J.; GAZZINELLI, G.; PELLEGRINO, J. & DIAS DA SILVA, W. - Schistosoma mansoni: the role of the complement of C₃ activating system in the cercaricidal action of normal serum. Exp. Parasit., 38: 20-29, 1975.
19. MELO, A.L. - Efeitos da oxamniquina sobre a transformaçăo da cercária de Schistosoma mansoni em esquistossômulo, in vivo Belo Horizonte, 1979. (Tese de Mestrado, Instituto de Cięncias Biológicas da UFMG).
20. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transformation in vivo. Rev. Soc. bras. Med. trop., 18: 17-21, 1985.
21. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transformation in vivo: 2. the effect of oxamniquine. Rev. Soc. bras. Med. trop., 18: 251-255, 1985.
22. MELO, A.L.; PEREIRA, L.H. & CHAMONE, M. - Sensibility of different larval stages of Schistosoma mansoni to the larvae disappearing reaction (LDR) in murine schistosomiasis. Rev. Inst. Med. trop. S. Paulo, 28: 395-399, 1986.
23. MELO, A.L.; PEREIRA, L.H. & CORREA, M.C.R. - In vivo cercaria-schistosomulum transformation: Inhibitory effect of oxamniquine. Trans. roy. Soc. trop. Med. Hyg., 72: 158-159, 1978.
24. MELO, A.L.; MACHADO, C.R.S. & PEREIRA, L.H. - Differences between in vitro and in vivo obtained schistosomules. Rev. Inst. Med. trop. S. Paulo, 32: 84-85, 1990.
25. PELLEGRINO, J. & MACEDO, D.G. - A simplified method for the concentration of cercariae. J. Parasit., 41: 329-330, 1955.
26. PELLEGRINO, J. & SIQUEIRA, A.F. - Técnica de perfusăo para colheita de Schistosoma mansoni em cobaias experimentalmente infectadas. Rev. bras. Malar., 8: 589-597, 1956.
27. PEREIRA, L.H.; PELLEGRINO, J.; VALADARES, T.E.; MELO, R.T. & COELHO, P.M.Z. - A new approach for screening prophylactic agents in schistosomiais. Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.
28. PEREIRA, L.H.; VALADARES, T.E.; CUNHA, M.L. & CORRĘA, M.C.R. - Recovery of young daughter sporocysts from snails infected with Schistosoma mansoni. Trans. roy. Soc. trop. Med. Hyg., 78: 563, 1984.
29. RAMALHO PINTO, F.J.; MCLAREN, D.J. & SMITHERS, S.R. - Complement mediated killing of schistosomula of Schistosoma mansoni by rat eosinophils in vitro. J. exp. Med., 147: 147-156, 1978.
30. RITCHIE, L.S. & BERRIOS-DURAND, L.A. - A simple procedure for recovering schistosome eggs in mass from tissues. J. Parasit., 47: 363-365, 1961.
31. ROSS, G.D. & MEDOF, M.E. - Membrane complement receptors specific for bound fragments of C₃ Advanc. Immunol., 37: 217-267, 1985.
32. RUPPEL, A. & CIOLI, D. - A comparative analysis of various developmental stages of Schistosoma mansoni with respect to their protein composition. Parasitology, 75: 339-343, 1977.
33. SADUN, E.H.; SCHOENBECHLER, M.J. & BENTZ, M. - Multiple antibody response in Schistosoma mansoni infections: antigenic constituents in eggs, cercariae and adults (excretions and secretions) determined by flocculation reactions, cross absorption and double diffusion studies. Amer. J. trop. Med. Hyg., 14: 977-995, 1965.
34. SAMUELSON, J.C. & CAULFIELD, J.P. - The cercarial glycocalyx of Schistosoma mansoni. J. Cell Biol., 100: 1423-1434, 1985.
35. SHER, A.; JAMES, S.L.; SIMPSON, A.J.G.; LAZDINS, J.K. & MELTZER, M.S. - Macrophages as effector cells of protective immunity in murine schistosomiasis. III. Loss of susceptibility to macrophagemediated killing during maturation of S. mansoni schistosomula from the skin to the lung stage. J. Immunol., 128: 1876-1879, 1982.
36. SNARY, D.; SMITH, M.A. & CLEGG, J.A. - Surface proteins of Schistosoma mansoni and their expression during morphogenesis. Europ. J. Immunol., 10: 573-575, 1980.
37. STANDEN, O.D. - The in vitro effect of normal and immune serum upon the cercariae of Schistosoma mansoni. J. Helminth., 26: 25-42, 1952.
38. WEISS, J.B. & STRAND, M. - Characterization of developmentally regulated epitopes of Schistosoma mansoni egg glycoprotein antigens. J. Immunol., 135: 1421-1429, 1985.
39. WEISS, J.B.; MAGNANI, J.L. & STRAND, M. - Identification of Schistosoma mansoni glycolipids that share immunogenic carbohydrate epitopes with glycoproteins. J. Immunol., 136: 4275-4282, 1986.
40. WRIGHT, S.D. & SILVERSTEIN, S.C. - Receptors for [complement] C₃b and [complement] C₃bi promote phagocytosis but not the release of toxic oxygen from human phagocytes. J. exp. Med., 158: 2016-2023, 1983.

Publication Dates

Publication in this collection
11 Sept 2006
Date of issue
June 1992

History

Accepted
11 Feb 1992
Received
26 Sept 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ANWAR, A.R.E.; SMITHERS, S.R. & KAY, A.B. - Killing of schistosomula of Schistosoma mansoni coated with antibody and/or complement by human leucocytes in vitro: requirement for complement in preferential killing by eosinophils. J. Immunol., 122: 628-637, 1979.

[2] 2. BUTTERWORTH, A.E.; TAYLOR, D.W.; VEITH, M.C.; VADAS, M.A.; DESSEIN, A.; STURROCK, R.F. & WELLS, E. - Studies on the mechanisms of immunity in human schistosomiasis. Immun. Rev., 61: 5-39, 1982.

[3] 3. CAPRON, A.; BIGUET, J.; ROSE, F. & VERNES, A. - Les antigčnes de Schistosoma mansoni II. Etude immunoéletrophorétique comparée de divers stades larvaires et des adultes des deux sexes. Aspects immunologiques des relations hôte-parasite de la cercaire et de l'adulte de S. mansoni. Ann. Inst. Pasteur, 109: 798-810, 1965.

[4] 4. CHAMONE, M.; MELO, A.L. & PEREIRA, L.H. - Effect of different saline solutions on the cercaricidal activity of normal human sera. Trans. roy. Soc. trop. Med. Hyg., 81: 147, 1987.

[5] 5. CHAIA, G. - Técnica para concentraçăo de miracídios. Rev. bras. Malar., 8: 355-357, 1956.

[6] 6. CULBERTSON, J.T. - The cercaricidal action of normal serums. J. Parasit., 22: 111-115, 1936.

[7] 7. CHIANG, C.P. & CAUFIELD, J.P. - Schistosoma mansoni: Ultrastructural demonstration of a miracidial glycocalyx that cross-reacts with antibodies raised against the cercarial glycocalyx. Exp. Parasit., 67: 63-72, 1988.

[8] 8. DALTON, J.P.; LEWIS, S.A.; ARONSTEIN, W.S. & STRAND, M. - Schistosoma mansoni: immunogenic glycoproteins of the cercarial glycocalyx. Exp. Parasit., 63: 215-226, 1987.

[9] 9. DIAS DA SILVA, W. & KAZATCHKINE, M.D. - Schistosoma mansoni activation of the alternative pathway of human complement by schistosomula. Exp. Parasit., 50: 278-286, 1980.

[10] 10. DUNNE, D.W. & BICKLE, Q.D. - Identification and characterization of a polysaccharide-containing antigen from Schistosoma mansoni eggs which cross-reacts with the surface of schistosomula. Parasitology, 94: 255-268, 1987.

[11] 11. DRESDEN, M.H. & DEELDER, A.M. - Schistosoma mansoni: Thiol proteinase properties of adult worm "hemoglobinase". Exp. Parasit., 48: 190-197, 1979.

[12] 12. EVELAND, L.K. - Schistosoma mansoni: conversion of cercariae to schistosomula. Exp. Parasit., 32: 261-264, 1972.

[13] 13. HARN, D.A.; QUINN, J.J.; CIANCI, C.M. & KO, A.I. - Evidence that protective membrane epitope is involved in early but not late phase immunity in Schistosoma mansoni. J. Immunol., 138: 1571-1580, 1987.

[14] 14. KEMP, W.M. - Ultrastructure of the cercarienhullen reaktion of Schistosoma mansoni. J. Parasit., 56: 713-723, 1970.

[15] 15. KEMP, W.M.; DAMIAN, R.T. & GREENE, N.D. - Schistosoma mansoni: Immunohistochemical location of CHR in glycocalyx of cercariae. Exp. Parasit., 33: 27-33, 1973.

[16] 16. KENT, N.H - Some Biochemical and immunological aspects of host-parasite relationships. Comparative immunochemisty of larval and adult forms of Schistosoma mansoni. Ann. N. Y. Acad. Sci., 113: 100-113, 1963.

[17] 17. LANDSPERGER, W.J.; STIREWALT, M.A. & DRESDEN, M.H. - Purification and properties of a proteolytic enzyme from the cercariae of the human trematode parasite Schistosoma mansoni. Biochem. J., 201: 137-144, 1982.

[18] 18. MACHADO, A.J.; GAZZINELLI, G.; PELLEGRINO, J. & DIAS DA SILVA, W. - Schistosoma mansoni: the role of the complement of C₃ activating system in the cercaricidal action of normal serum. Exp. Parasit., 38: 20-29, 1975.

[19] 19. MELO, A.L. - Efeitos da oxamniquina sobre a transformaçăo da cercária de Schistosoma mansoni em esquistossômulo, in vivo Belo Horizonte, 1979. (Tese de Mestrado, Instituto de Cięncias Biológicas da UFMG).

[20] 20. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transformation in vivo. Rev. Soc. bras. Med. trop., 18: 17-21, 1985.

[21] 21. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transformation in vivo: 2. the effect of oxamniquine. Rev. Soc. bras. Med. trop., 18: 251-255, 1985.

[22] 22. MELO, A.L.; PEREIRA, L.H. & CHAMONE, M. - Sensibility of different larval stages of Schistosoma mansoni to the larvae disappearing reaction (LDR) in murine schistosomiasis. Rev. Inst. Med. trop. S. Paulo, 28: 395-399, 1986.

[23] 23. MELO, A.L.; PEREIRA, L.H. & CORREA, M.C.R. - In vivo cercaria-schistosomulum transformation: Inhibitory effect of oxamniquine. Trans. roy. Soc. trop. Med. Hyg., 72: 158-159, 1978.

[24] 24. MELO, A.L.; MACHADO, C.R.S. & PEREIRA, L.H. - Differences between in vitro and in vivo obtained schistosomules. Rev. Inst. Med. trop. S. Paulo, 32: 84-85, 1990.

[25] 25. PELLEGRINO, J. & MACEDO, D.G. - A simplified method for the concentration of cercariae. J. Parasit., 41: 329-330, 1955.

[26] 26. PELLEGRINO, J. & SIQUEIRA, A.F. - Técnica de perfusăo para colheita de Schistosoma mansoni em cobaias experimentalmente infectadas. Rev. bras. Malar., 8: 589-597, 1956.

[27] 27. PEREIRA, L.H.; PELLEGRINO, J.; VALADARES, T.E.; MELO, R.T. & COELHO, P.M.Z. - A new approach for screening prophylactic agents in schistosomiais. Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.

[28] 28. PEREIRA, L.H.; VALADARES, T.E.; CUNHA, M.L. & CORRĘA, M.C.R. - Recovery of young daughter sporocysts from snails infected with Schistosoma mansoni. Trans. roy. Soc. trop. Med. Hyg., 78: 563, 1984.

[29] 29. RAMALHO PINTO, F.J.; MCLAREN, D.J. & SMITHERS, S.R. - Complement mediated killing of schistosomula of Schistosoma mansoni by rat eosinophils in vitro. J. exp. Med., 147: 147-156, 1978.

[30] 30. RITCHIE, L.S. & BERRIOS-DURAND, L.A. - A simple procedure for recovering schistosome eggs in mass from tissues. J. Parasit., 47: 363-365, 1961.

[31] 31. ROSS, G.D. & MEDOF, M.E. - Membrane complement receptors specific for bound fragments of C₃ Advanc. Immunol., 37: 217-267, 1985.

[32] 32. RUPPEL, A. & CIOLI, D. - A comparative analysis of various developmental stages of Schistosoma mansoni with respect to their protein composition. Parasitology, 75: 339-343, 1977.

[33] 33. SADUN, E.H.; SCHOENBECHLER, M.J. & BENTZ, M. - Multiple antibody response in Schistosoma mansoni infections: antigenic constituents in eggs, cercariae and adults (excretions and secretions) determined by flocculation reactions, cross absorption and double diffusion studies. Amer. J. trop. Med. Hyg., 14: 977-995, 1965.

[34] 34. SAMUELSON, J.C. & CAULFIELD, J.P. - The cercarial glycocalyx of Schistosoma mansoni. J. Cell Biol., 100: 1423-1434, 1985.

[35] 35. SHER, A.; JAMES, S.L.; SIMPSON, A.J.G.; LAZDINS, J.K. & MELTZER, M.S. - Macrophages as effector cells of protective immunity in murine schistosomiasis. III. Loss of susceptibility to macrophagemediated killing during maturation of S. mansoni schistosomula from the skin to the lung stage. J. Immunol., 128: 1876-1879, 1982.

[36] 36. SNARY, D.; SMITH, M.A. & CLEGG, J.A. - Surface proteins of Schistosoma mansoni and their expression during morphogenesis. Europ. J. Immunol., 10: 573-575, 1980.

[37] 37. STANDEN, O.D. - The in vitro effect of normal and immune serum upon the cercariae of Schistosoma mansoni. J. Helminth., 26: 25-42, 1952.

[38] 38. WEISS, J.B. & STRAND, M. - Characterization of developmentally regulated epitopes of Schistosoma mansoni egg glycoprotein antigens. J. Immunol., 135: 1421-1429, 1985.

[39] 39. WEISS, J.B.; MAGNANI, J.L. & STRAND, M. - Identification of Schistosoma mansoni glycolipids that share immunogenic carbohydrate epitopes with glycoproteins. J. Immunol., 136: 4275-4282, 1986.

[40] 40. WRIGHT, S.D. & SILVERSTEIN, S.C. - Receptors for [complement] C₃b and [complement] C₃bi promote phagocytosis but not the release of toxic oxygen from human phagocytes. J. exp. Med., 158: 2016-2023, 1983.

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Schistosoma mansoni: host cell adhesion to the different stages of the parasite, in vivo

Schistosoma mansoni: adesão de células do hospedeiro aos diferentes estádios do parasito, in vivo

Abstracts

Publication Dates

History