ABSTRACT
The advancement of biomaterials technology allows the development of materials that assist in reconstructive procedures of the body parts and increase treatment and improves the humans quality of life. The polyhydroxybutyrate (PHB) is a biocompatible polymer, biodegradable, widely studied for medical applications and its properties can also be suitable for other various applications with hydroxyapatite (HA) incorporation. This is a bioactive and biocompatible inorganic filler already present in bone tissues. Thus, this study aimed to obtain membranes composed of PHB and HA for use as biomaterials in bone tissue regeneration and guided. To obtain the membranes, PHB was processed in a single screw extruder AX-Plastics bench (16 mm), with LD Ratio = 26, a compression ratio of 1.5, specific for the production of flat film. Pure PHB membranes were prepared, and composite PHB with 1%, 2% and 3% HA. After obtaining the membranes, they were characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) / Energy Dispersive Spectroscopy (EDS) and Cytotoxicity. In the diffraction profile it was observed a semicrystalline material and the addition of HA decreased this profile. In the micrographs it was visualized a surface of a dense and rough material. The EDS evidenced the chemical elements present in the PHB and HA. The cytotoxicity assay confirmed the viability and thus the biocompatibility of the material. The Wettability test confirmed the hydrophilic character of the membranes. Based on these results, we can conclude that the method for obtaining the composite was effective, being biocompatible and the charge amount will depend on the biomaterial usage indication concerned.
Keywords:
PHB; Hydroxyapatite; Biomaterials; Biocompatible
