Determinação de Di(2-etilhexil ftalato) liberado no sangue por linhas de PVC durante o procedimento de hemodiálise

Fernandes, Beatriz Luci; Maria, Fernanda; Nakao, Lia Sumie; Ingberman, Max; Cubas, Marcia Regina

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Sumário

Artigo • Matéria (Rio J.) 23 (1) • 2018 • https://doi.org/10.1590/S1517-707620170001.0282 copiar

Determinação de Di(2-etilhexil ftalato) liberado no sangue por linhas de PVC durante o procedimento de hemodiálise

Autoria SCIMAGO INSTITUTIONS RANKINGS

RESUMO

O objetivo deste trabalho é demonstrar a aplicabilidade da Cromatografia Líquida de Alta Eficiência na quantificação do Di(2-etilhexil ftalato) (DEHP) liberado pela linha de Policloreto de vinila (PVC) no sangue circulante durante o procedimento de hemodiálise. Como a proposta não é a validação do método foram coletadas amostras de sangue de cinco pacientes voluntários e de três voluntários saudáveis e os plasmas foram isolados, preparados e analisados. A concentração média de DEHP no sangue no plasma dos pacientes foi 0.19±0.12 mg/kg de massa corpórea, enquanto que no plasma dos voluntários saudáveis foi 0.003±0.002 µg/kg de massa corpórea. Os resultados mostraram que o método apresentado é uma alternativa para a determinação de DEPH no sangue e que poderá auxiliar na busca por outros materiais para aplicação em equipamentos de circulação extracorpórea. O estudo foi realizado de acordo com os padrões nacionais e internacionais de ética em pesquisa envolvendo seres humanos (CEP PUCPR Prot. n.5802).

Palavras-chave:
Diálise renal; Toxicidade crônica; DEHP

Patient/Session	Reuse number	Heparin dose in the circuit (UI)	Blood flow (ml/min)	Period of hemodialysis therapy
P1/1 P1/2 P1/3	0 2 4	5,000	300	3 years and 3 months
P2/1 P2/2 P2/3	5 7 9	without heparin^* * Patient candidate for renal transplant. Reuse number 0 means first use.	300	5 years
P3/1 P3/2 P3/3	9 11 13	6,000	300	3 year and 6 months
P4/1 P4/2 P4/3	0 2 4	7,000	350	4 years and 4 months
P5/1 P5/2 P5/3	11 13 15	8,000	350	4 years and 9 months

Day of analysis	Linear regression	Determination coefficient (r²)	Volunteer / session	Area under the peak (x)
1	y=3.10⁻⁸x – 4.10⁻⁵	0.9974	P4 / 1 initial P4 /1 final P4 / 2 initial P4 / 2 final P4 / 3 initial P4 / 3 final P5 / 1 initial P5 / 1 final P5 / 2 initial P5 / 2 final	3,576.0 20,223.8 2,332.8 2,480.0 4,931.2 6,680.0 34,129.6 43,718.4 7,603.2 7,740.8
2	y=2.10⁻⁸x + 2.10⁻⁵	0.9990	P5 / 3 initial P5 / 3 final	3,803.2 23,950.4
3	y=2.10⁻⁸x + 6.10⁻⁶	0.9913	P1 / 1 initial P1 / 1 final P1 / 2 initial P1 / 2 final P1 / 3 initial P1 / 3 final	5,817.6 6,905.6 6,240.0 6,649.6 1,292.8 4,763.2
4	y= 3.10⁻⁸x+ 8.10⁻⁵	0.9954	P2 / 1 initial P2 / 1 final P2 / 2 initial P2 / 2 final P2 / 3 initial P2 / 3 final	6,428.8 14,163.2 5,017.6 12,294.4 7,878.4 8,601.6
5	y= 3.10⁻⁸ x + 2.10⁻⁵	0.9989	P3 / 1 initial P3 / 1 final P3 / 2 initial P3 / 2 final P3 / 3 initial P3 / 3 final	8,726.4 9,568.1 6,364.8 10,486.4 4,528.8 9,740.8
6	y= 2.10⁻⁸ x – 2.10⁻⁵	0.9994	V1 V2 V3	2,750.4 1,081.6 1,604.8

Patient/session	Concentration of DEHP (μg/ml) 15 min after the session has started	Concentration of DEHP (μg/ml) 15 min before the session has finished	DEHP accumulated per patient, ΔDEHP_m (μg/ml) ± SD	VC (%) for ΔDEHP_m
P1 / 1 P1 / 2 P1 / 3	0.122^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.131^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.032^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.144^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.139^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.101^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.033±0.030	96,83
P2 / 1 P2 / 2 P2 / 3	0.273 0.231 0.316	0.505 0.449 0.338	0.157±0.120	74.63
P3 / 1 P3 / 2 P3 / 3	0.242 0.171^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.101^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.267 0.296 0.202	0.083±0.252	62.40
P4 / 1 P4 / 2 P4 / 3	0.103^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.029^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.108^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.567 0.034^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.161^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.174±0.231	144,99
P5 / 1 P5 / 2 P5 / 3	0.984 0.188^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.096^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	1.272 0.192^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.499	0.232±0.205	88.64

Patient/Session	ΔDEHP (μg/ml)	Blood flow (ml/min)	ΔDEHP x flow (µg/min)	MDEHP per session (mg)	MDEHPm (mg) ± SD
P1 / 1 P1 / 2 P1 / 3	0.022 0.008 0.069	300	6.60 2.40 20.70	– – –	–
P2 / 1 P2 / 2 P2 / 3	0.232 0.218 0.022	300	69.60 65.40 6.60	16.70 15.69 1.58	11.32±8.45
P3 / 1 P3 / 2 P3 / 3	0.025 0.125 0.053	300	7.50 37.50 15.90	1.80 - -	–
P4 / 1 P4 / 2 P4 / 3	0.464 0.005 0.523	350	162.40 1.75 183.05	– – –	–
P5 / 1 P5 / 2 P5 / 3	0.287 0.004 0.403	350	100.45 1.40 141.05	24.11 - -	–

Patient	MDEHP_m (mg)	Body weight (kg)	MDEHP m/K (mg/kg)
P2	11.32±8.45	60.0±0.05	0.19±0.14
P3	1,80±0,00	71.5±0.05	0.07±0.05
P4	27.78±23.81	70.0±0.05	0.39±0.34
P5	19.43±17.24	75.5±0.05	0.26±0.23

Volunteer	DEHP (μg/ml)	MDEHPm (μg)	Body weight	DEHPm (μg/kg)
V1	0.035	0.35	60.20±0.05	0.006±0,001
V2	0.002	0.02	54.50±0.05	0.000±0.001
V3	0.012	0.12	56.40±0.05	0.002±0.001

Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiro, em cooperação com a Associação Brasileira do Hidrogênio, ABH2 Av. Moniz Aragão, 207, 21941-594, Rio de Janeiro, RJ, Brasil, Tel: +55 (21) 3938-8791 - Rio de Janeiro - RJ - Brazil
E-mail: revmateria@gmail.com

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[1] Autor Responsável: Beatriz Luci Fernandes