Abstract
Compact and non-homogeneous callus were induced to start from internodal explant of Datura insignis Barb. Rodr. Cultured on B5 medium (Gamborg et al., 1968) supplemented with 1mg/l BAP + 0,25 and 1mg/l NAA. The callus histological analysis showed that although periclinal division may have occurred in the epidermis, colenchyma and cortical, interfascicular and medullary parenchyma, only the tissues situated between the epidermis and central cylinder were transformed into callogenic tissue. It was observed the differentiation of xilematic elements and meristematic centers dispersed by callus. On a medium with 1 mg/l BAP + 0,25mg NAA was observed the presence of globular structures made up of cells in periclinal division with radial array and many meristematic centers. We could not identify the presence of somatic embryos if one considers the callus as not embryogenic.