Abstract
Plant tissue culture is essential to the uniform propagation and multiplication of plants, to the germplasm conservation, to the genetic improvement programs and genetic transformation. This technique has been required more studies for the understanding of the mechanisms involved in microbial growth in culture media. As well as the relationships established with the host plant. Therefore, the present revision intends to clarify these questionings and to promote the distinction between contamination and endophytic manifestation which occur in vitro culture for different causes. This distinction allows to reduce the panic installed when the microorganisms appear, besides helping to adopt measures of prevention and /or control of these events avoiding unnecessary discards of material with high commercial and genetic value.
Key words:
plant tissue culture; unnecessary discard; endophytes; microorganisms
