Objective
To evaluate the dentin effect on pH and antimicrobial activity of the calcium hydroxide chemical formulations.
Material and method
pH and antimicrobial activity of calcium hydroxide formulations were evaluated (G1-polyethyleneglycol, G2-glycerin and camphorated paramonochlorophenol and G3-chlorhexidine). The formulations were evaluated alone (G1A, G2A e G3A) or with dentin powder (G1B, G2B and G3B). Polyethylene tubes were filled with the formulation and immersed in distilled water. After 24 hours, 7, 14 and 21 days, pH was evaluated. The antimicrobial activity was evaluated against Enterococcus faecalis by agar diffusion test. All data were submitted to ANOVA and Tukey tests (p=0.05).
Result
None effect was observed on pH and antimicrobial activity by dentin on different chemical formulations, except the reduction occurred in G1 at 24 hours (p <0.05). When compared between the groups at 24 hours and 7 days, G1A and G1B had a lower pH (p <0.05). At 14 days, G2A, G2B, G3A and G3B showed similar pH (p <0.05) and between G3B and G1A (p> 0.05). At 21 days, all formulations were similar (p> 0.05). Regarding antimicrobial activity G2B G2A => = G3A G3B> = G1A G1B (p <0.05).
Conclusion
The dentin did not affect the pH and antimicrobial activity of different formulations containing calcium hydroxide, except in association with polyethylene glycol, which caused the reduction of pH, within 24 hours. The formulation with paramonochlorophenol camphor showed higher antimicrobial activity, followed by chlorhexidine and polyethylene glycol.
Calcium; endodontic; Enterococcus faecalis ; pH