Maternal schistosomiasis: IL-2, IL-10 and regulatory T lymphocytes to unrelated antigen in adult offspring mice

We used animals born (BIM) or suckled (SIM) from infected mothers; and mice born/suckled from infected (BSIM) or non-infected mothers (CONTROL). After OA+adjuvant immunization, spleen cells were cultured, with or without OA, and doubly marked for cytometry.

RESULTS:

BIM showed fewer CD4+/IL-2+ and more B220+/IL-10+ cells, whereas the SIM group showed increased Treg frequency. BSIM had fewer B220+/IL-10+ and Treg cells.

CONCLUSIONS:

Separately, gestation or nursing induced immunosuppressive cells in infected mothers, but improved anti-OA immunity when combined.

Keywords:
Maternal schistosomiasis; Adult offspring; Immunomodulation

Authorship

Erica de Souza Fernandes

Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil. Universidade Federal de PernambucoBrazilRecife, PE, BrazilLaboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil.

Virgínia Maria de Barros Lorena

Instituto Aggeu Magalhães, Laboratório de Imunologia, Fundação Oswaldo Cruz, Recife, PE, Brasil. Fundação Oswaldo CruzBrazilRecife, PE, BrazilInstituto Aggeu Magalhães, Laboratório de Imunologia, Fundação Oswaldo Cruz, Recife, PE, Brasil.

Iana Rafaela Fernandes Sales

Mônica Camelo Pessoa de Azevedo Albuquerque

Departamento de Medicina Tropical, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, PE, Brasil. Universidade Federal de PernambucoBrazilRecife, PE, BrazilDepartamento de Medicina Tropical, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, PE, Brasil.

Yara de Miranda Gomes

Vlaudia Maria Assis Costa

Valdênia Maria Oliveira de Souza

Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, PE, Brasil.Universidade Federal de PernambucoBrazilRecife, PE, BrazilDepartamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, PE, Brasil.

Corresponding author: Drª Valdênia Maria Oliveira de Souza. e-mail:valdenia.souza@gmail.com

Conflict of interest: The authors declare that there is no conflict of interest.

SCIMAGO INSTITUTIONS RANKINGS

Figures

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FIGURE 1:
Frequency of CD4+ and IL-2+(A) or FoxP3+(B) cells in adult mice born (BIM) or suckling in infected mothers (SIM); mice born and suckling in infected (BSIM) or non-infected mothers (CONTROL) when immunized with OA+CFA. 5 × 10⁵ cells cultured with OA (500µg/ml), or not, were labeled with FITC anti-CD4 and PerCP anti-IL2 (A) or PE anti-FoxP3 (B). SIM: suckling in infected mothers; BSIM: born and suckling from infected mothers; BIM: born from infected mothers; CONTROL: non-infected mothers; OA: ovalbumin; CD4+: cluster of differentiation 4+; IL: interleukin; CFA: complete Freund’s adjuvant; FITC: fluoresceína isotiocianato; PE: phycoerythrin. *p <0.05 when compared to respective CONTROL groups.

Thumbnail

FIGURE 2:
Frequency of CD3+(A), B220+(B), or CD14+(C) cells, and IL-10+ in adult mice born (BIM) or suckling in infected mothers (SIM); mice born and suckling in infected (BSIM) or non-infected mothers (CONTROL) when immunized with OA in CFA. 5 × 10⁵ cells cultured with OA (500µg/ml), or not, were labeled with PE-Cy5 anti-CD3(A), FITC anti-B220(B), or FITC anti-CD14(C), and PE anti-IL-10. SIM: suckling in infected mothers; BSIM: born and suckling from infected mothers; BIM: born from infected mothers; CONTROL: non-infected mothers; CD: cluster of differentiation; IL: interleukin; OA: ovalbumin; CFA: complete Freund’s adjuvant; FITC: fluoresceína isotiocianato; PE: phycoerythrin. *p <0.05 when compared to non-stimulated cells from BIM groups.

FIGURE 1: Frequency of CD4+ and IL-2+(A) or FoxP3+(B) cells in adult mice born (BIM) or suckling in infected mothers (SIM); mice born and suckling in infected (BSIM) or non-infected mothers (CONTROL) when immunized with OA+CFA. 5 × 10⁵ cells cultured with OA (500µg/ml), or not, were labeled with FITC anti-CD4 and PerCP anti-IL2 (A) or PE anti-FoxP3 (B). SIM: suckling in infected mothers; BSIM: born and suckling from infected mothers; BIM: born from infected mothers; CONTROL: non-infected mothers; OA: ovalbumin; CD4+: cluster of differentiation 4+; IL: interleukin; CFA: complete Freund’s adjuvant; FITC: fluoresceína isotiocianato; PE: phycoerythrin. *p <0.05 when compared to respective CONTROL groups.

FIGURE 2: Frequency of CD3+(A), B220+(B), or CD14+(C) cells, and IL-10+ in adult mice born (BIM) or suckling in infected mothers (SIM); mice born and suckling in infected (BSIM) or non-infected mothers (CONTROL) when immunized with OA in CFA. 5 × 10⁵ cells cultured with OA (500µg/ml), or not, were labeled with PE-Cy5 anti-CD3(A), FITC anti-B220(B), or FITC anti-CD14(C), and PE anti-IL-10. SIM: suckling in infected mothers; BSIM: born and suckling from infected mothers; BIM: born from infected mothers; CONTROL: non-infected mothers; CD: cluster of differentiation; IL: interleukin; OA: ovalbumin; CFA: complete Freund’s adjuvant; FITC: fluoresceína isotiocianato; PE: phycoerythrin. *p <0.05 when compared to non-stimulated cells from BIM groups.

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Maternal schistosomiasis: IL-2, IL-10 and regulatory T lymphocytes to unrelated antigen in adult offspring mice

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[1] Corresponding author: Drª Valdênia Maria Oliveira de Souza. e-mail:valdenia.souza@gmail.com