Abstract
INTRODUCTION:
In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia (“La Balsa”).
METHODS:
RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bayesian inference using reference DENV-2 sequences were performed.
RESULTS:
Twelve pools that tested positive for DENV-2 were characterized based on the C-PrM region and grouped under the Asian/American clade.
CONCLUSIONS:
This study is the first to report the DENV-2 Asian-American subtype in a rural area of Cordoba region, which is associated with severe dengue and local epidemics.
Keywords:
DENV-2; Genotypes; Phylogenetic; Aedes aegypti; Colombia