Serological and molecular detection of Leptospira spp in dogs

Abstract

INTRODUCTION:

This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs.

METHODS:

Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively.

RESULTS:

Six (5.7%) and one (1%) animals were positive by MAT and PCR, respectively.

CONCLUSIONS:

These results show a low prevalence of infection by Leptospira spp. The absence of positive results for the Icterohaemorrhagiae serogroup indicates the small relevance of these dogs as sources of human leptospirosis.

Keywords:
Leptospirosis; PCR; Microscopic; Agglutination test