In vivo antileishmanial activity of Annona mucosa extracts

Lima, Janaína Paolucci Sales de; Pinheiro, Maria Lúcia Belém; Allaman, Ivan Bezerra; Silva-Jardim, Izaltina

doi:10.1590/0037-8682-0139-2019

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Short Communication • Rev. Soc. Bras. Med. Trop. 53 • 2020 • https://doi.org/10.1590/0037-8682-0139-2019 linkcopy

In vivo antileishmanial activity of Annona mucosa extracts

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION: Leishmaniasis, a disease caused by a parasite endemic to large areas of tropical and subtropical countries, is a growing public health problem.

METHODS: Male BALB/c mice were infected with Leishmania amazonensis and treated with extracts isolated from Annona mucosa.

RESULTS: Treated groups had significantly reduced footpad swelling. The group treated intraperitoneally with hexane extract showed footpad swelling similar to groups treated with Pentamidine® and Glucantime®. Groups treated with dichloromethane extract and hexane extract presented the recovering phenotype associated with reduced parasite levels.

CONCLUSIONS: Extracts of A. mucosa are promising sources of novel antileishmanial compounds.

Keywords: Leishmaniasis; Leishmania amazonenses; Chemotherapy

Authorship

Janaína Paolucci Sales de Lima

Universidade Federal do Amazonas, Departamento de Produção Animal e Vegetal, Manaus, AM, Brasil. Universidade Federal do AmazonasBrasilManaus, AM, Brasil Universidade Federal do Amazonas, Departamento de Produção Animal e Vegetal, Manaus, AM, Brasil.

https://orcid.org/0000-0003-3771-3891

Maria Lúcia Belém Pinheiro

Universidade Federal do Amazonas, Departamento de Química, Manaus, AM, Brasil. Universidade Federal do AmazonasBrasilManaus, AM, Brasil Universidade Federal do Amazonas, Departamento de Química, Manaus, AM, Brasil.

Ivan Bezerra Allaman

Universidade Estadual de Santa Cruz, Departamento de Ciências Exatas e Tecnológicas, Ilhéus, BA, Brasil. Universidade Estadual de Santa CruzBrasilIlhéus, BA, Brasil Universidade Estadual de Santa Cruz, Departamento de Ciências Exatas e Tecnológicas, Ilhéus, BA, Brasil.

Izaltina Silva-Jardim

Universidade Estadual de Santa Cruz, Departamento de Ciências Biológicas, Ilhéus, BA, Brasil. Universidade Estadual de Santa CruzBrasilIlhéus, BA, Brasil Universidade Estadual de Santa Cruz, Departamento de Ciências Biológicas, Ilhéus, BA, Brasil.

Corresponding author: Drª. Janaína Paolucci Sales de Lima. e-mail:paolucci@ufam.edu.br

Authors’ contribution: JL: contributed in collecting plant sample and identification, confection of herbarium, running the laboratory work, analysis of the data and drafted the paper. MLP: contributed to chromatographic analysis. ISJ: contributed to biological studies. MLP and ISJ: designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. IA: contributed analysis of the data.

Conflict of Interest: The authors declare that there is no conflict of interest.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (3)

Thumbnail

FIGURE 1:
Variability of the animals as a function of time for the footpad swelling and body weight variables during the pre-treatment. The footpad swelling describes the difference between infected and non-infected footpad. The body weight variation describes the loss of weight in relation to the day of infection between the final weight and starting weight of individual mice. (A): Footpad swelling in pre-treatment. (B): Body weight in pre-treatment.

Thumbnail

FIGURE 2:
Footpad swelling and body weight variables of the different groups during the 7 (A,C) and 14^th (B,D) experimental weeks. Group 1 - Control, Group 2 - Lanette Cream®, Group 3 - Pentamidine®, Group 4 - Glucantime®, Group 5 - Dichloromethane extract cream, Group 6 - Hexane extract cream, Group 7 - Dichloromethane extract, and Group 8 - Hexane extract. Means with distinct letters differ significantly (Scott-Knott test, P <0.05). Vertical bars represent the 95% confidence interval. Averages were adjusted based on covariates of the last week prior to treatment.

Thumbnail

FIGURE 3:
Footpad swelling (A,B) body weight (C), and parasite load (D) post-treatment of the different groups. Group 1 - Control, Group 2 - Lanette Cream®, Group 3 - Pentamidine®, Group 4 - Glucantime®, Group 5 - Dichloromethane extract cream, Group 6 - Hexane extract cream, Group 7 - Dichloromethane extract and Group 8 - Hexane extract. Means with distinct letters differ significantly (Scott-Knott test, P <0.05). Vertical bars represent the 95% confidence interval. Averages were adjusted based on the covariates of the last week before the experiment (A,B,C). (E) Photographic documentation of the infected footpads of three exemplary mice per group, revealing the severity of disease progression in the treated and untreated groups nine weeks post-infection.

imageFIGURE 1: Variability of the animals as a function of time for the footpad swelling and body weight variables during the pre-treatment. The footpad swelling describes the difference between infected and non-infected footpad. The body weight variation describes the loss of weight in relation to the day of infection between the final weight and starting weight of individual mice. (A): Footpad swelling in pre-treatment. (B): Body weight in pre-treatment. open_in_new

imageFIGURE 2: Footpad swelling and body weight variables of the different groups during the 7 (A,C) and 14^th (B,D) experimental weeks. Group 1 - Control, Group 2 - Lanette Cream®, Group 3 - Pentamidine®, Group 4 - Glucantime®, Group 5 - Dichloromethane extract cream, Group 6 - Hexane extract cream, Group 7 - Dichloromethane extract, and Group 8 - Hexane extract. Means with distinct letters differ significantly (Scott-Knott test, P <0.05). Vertical bars represent the 95% confidence interval. Averages were adjusted based on covariates of the last week prior to treatment. open_in_new

imageFIGURE 3: Footpad swelling (A,B) body weight (C), and parasite load (D) post-treatment of the different groups. Group 1 - Control, Group 2 - Lanette Cream®, Group 3 - Pentamidine®, Group 4 - Glucantime®, Group 5 - Dichloromethane extract cream, Group 6 - Hexane extract cream, Group 7 - Dichloromethane extract and Group 8 - Hexane extract. Means with distinct letters differ significantly (Scott-Knott test, P <0.05). Vertical bars represent the 95% confidence interval. Averages were adjusted based on the covariates of the last week before the experiment (A,B,C). (E) Photographic documentation of the infected footpads of three exemplary mice per group, revealing the severity of disease progression in the treated and untreated groups nine weeks post-infection. open_in_new

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Sociedade Brasileira de Medicina Tropical - SBMT Sociedade Brasileira de Medicina Tropical - SBMT, Núcleo de Medicina Tropical UnB, Sala 43C 70904-970, E-mails: rsbmt@uftm.edu.br | artes.rsbmt@gmail.com | sbmt@sbmt.org.br , WhatsApp: SBMT (61) 9.9192-6496, WhatsApp: RSBMT (34) 9.9996-5807 - Brasília - DF - Brazil
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