Acessibilidade / Reportar erro
Short Communication • Rev. Soc. Bras. Med. Trop. 50 (02) • Mar-Apr 2017 • https://doi.org/10.1590/0037-8682-0364-2016   copy

A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.

METHODS:

Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.

RESULTS:

The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.

CONCLUSIONS:

Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.

Keywords:
Leptospira; Leptospirosis; Duplex endpoint; PCR

Sociedade Brasileira de Medicina Tropical - SBMT Caixa Postal 118, 38001-970 Uberaba MG Brazil, Tel.: +55 34 3318-5255 / +55 34 3318-5636/ +55 34 3318-5287, http://rsbmt.org.br/ - Uberaba - MG - Brazil
E-mail: rsbmt@uftm.edu.br
Acompanhe os números deste periódico no seu leitor de RSS