A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Abstract

INTRODUCTION:

This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.

METHODS:

Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.

RESULTS:

The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 10³ leptospires/ml in both spiked urine and water.

CONCLUSIONS:

Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.

Keywords:
Leptospira; Leptospirosis; Duplex endpoint; PCR