In vivo and in vitro activity of oil extract of garlic (Allium sativum Linnaeus) against Schistosoma japonicum cercariae

Wan, Kang; Wang, Peng; Zhang, Leibo

doi:10.1590/0037-8682-0301-2016

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Revista da Sociedade Brasileira de Medicina Tropical

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Short Communication • Rev. Soc. Bras. Med. Trop. 50 (01) • Jan-Feb 2017 • https://doi.org/10.1590/0037-8682-0301-2016 copy

In vivo and in vitro activity of oil extract of garlic (Allium sativum Linnaeus) against Schistosoma japonicum cercariae

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract:

INTRODUCTION:

The activity of garlic oil extract against Schistosoma japonicum cercariae was evaluated.

METHODS:

The in vitro and in vivo cercaricidal activities against S. japonicum larvae were determined.

RESULTS:

Exposure to ≥ 10^-6 (v/v) garlic emulsions for 30 min led to 100% cercariae mortality; pre-exposure treatment with ≥ 10^-4 (v/v) garlic emulsions showed 100% preventive efficacy against S. japonicum infection, while pre-treatment with 10^-5 and 10^-6 (v/v) emulsions achieved 20%-40% preventive efficacy and 35.2%-63.6% worm burden reduction.

CONCLUSIONS:

Garlic oil extract has activity against S. japonicum larvae and a promising preventive efficacy against S. japonicum infection.

Keywords:
Schistosoma japonicum; Allium sativum; Cercaricidal activity

Treatment*	Mortality of cercariae (mean ± SD, %)
	1 min	30 min	1h	4h
10^-2 garlic oil extract	100.0	100.0	100.0	100.0
10^-3 garlic oil extract	100.0	100.0	100.0	100.0
10^-4 garlic oil extract	98.7 ± 0.8	100.0	100.0	100.0
10^-5 garlic oil extract	14.7 ± 3.8	100.0	100.0	100.0
10^-6 garlic oil extract	7.8 ± 2.1	100.0	100.0	100.0
Tween-80	0.0	0.0	1.1 ± 0.1	3.2 ± 1.3
Dechlorinated water	0.0	0.0	0.0	1.3 ± 0.2

Pre-exposure treatment*	Experiment 1			Experiment 2
	infection	mean worm	worm	infection	mean worm	worm
	rate (%)	burden per	burden	rate (%)	burden per	burden
		mouse	reduction		mouse	reduction
		(mean ± SD)	(%)		(mean ± SD)	(%)
10^-2 garlic oil extract	0.0	0	100.0	0.0	0	100.0
10^-3 garlic oil extract	0.0	0	100.0	0.0	0	100.0
10^-4 garlic oil extract	0.0	0	100.0	0.0	0	100.0
10^-5 garlic oil extract	60.0	14.1 ± 4.1	62.1	70.0	13.8 ± 2.7	63.6
10^-6 garlic oil extract	80.0	24.1 ± 3	35.2	80.0	23.2 ± 2.8	38.8
Tween-80	100.0	35.1 ± 3.7	5.6	100.0	36.3 ± 4.5	4.2
Dechlorinated water	100.0	37.2 ± 2.9	-	100.0	37.9 ± 1.9	-

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[1] Corresponding author: Dr. Leibo Zhang. e-mail: sunshinewang724@163.com