This work was carried out to test and to select wild strains of Trichoderma spp. with antagonistic ability against Verticillium dahliae. The in vitro assay consisted of the antibiosis technique by assessment of volatile and non-volatile metabilites and hyphal interaction. Forty seven Trichoderma strains were evaluated and twenty strains were selected by their antagonistic ability against V. dahliae. Through the antibiosis test, seven strains of different species T15P and Tal-1 (T. viride); TW6 and CNP311A (T. koningii); CNP17 and TCII (T. harzianum) and Tal-10 (T. aureoviride) inhibited the mycelial growth of V. dahliae due to the production of metabolites. Several kinds of hyphal interference were observed such as coiling and hook formation.
biocontrol; parasistism; Trichoderma; Verticillium; antagonism