The object of this study was to find a nutricional means for the cultivation of the shoot tips of Perola pineapples, in order to free them of infections caused by Fusarium subglutinas. The first step was to remove healthy shoot tips from healthy crowns under aseptic conditions and inoculate nine different cultivation media with or whithout traces of BAP, GA3 and AIA. The levels of necrosis in the plants were studied after 30,45 and 60 days. Selected shoot tips removed from the plants contaminated with F. subglutinans were inoculated to check their viability as pathogen-free clones. The results revealed the influence of the cultivation media in the regeneration of plants and the viability of clonal cleansing of infected plants. It was also noted that 100% of the regenerated plants remained pathogen-free.
Ananas comosus; pineapple; culture medium; shoot tip
