Acessibilidade / Reportar erro

Use of bulked segregant analysis in identification of molecular markers linked to resistance to rust (Puccinia psidii winter) in Eucalyptus sp.

Due to the great importance of the Eucalyptus crop in Brazil, companies in the forest sector have aimed, through genetic breeding programs, to reduce yield losses and meet the demands of the paper and cellulosis market. One example is the search for genes for resistance to diseases, especially the rust caused by Puccinia psidii Winter, which results in reduced productivity in highly susceptible plants. On the present study, seedlings of Eucalyptus from an F1 generation, bred from controlled crossing between parents C0 (resistant) and VR (susceptible), were innoculated with Puccinia psidii in a greenhouse and followed until the rust symptoms appeared. They were classified into two groups, according to their reaction in: resistant (absence of symptoms) and susceptible (presence of symptoms and buds), whose DNA samples were compared using mollecular markers associated to the BSA (Bulked Segregant Analysis) method. The polymorphism among the "bulks" was genetically characteristic. Among the 720 tested "primers", 19 were polymorphic. But, only the AK01 marker kept present, when tested in all individuals who composed the "bulk" and in the rest of the population and it was shown as located in an estimated genetic distance of 20 cM repulsion from the resistance gene.

Eucalyptus sp.; Puccinia psidii; resistence; Bulked Segregant Analysis


Grupo Paulista de Fitopatologia FCA/UNESP - Depto. De Produção Vegetal, Caixa Postal 237, 18603-970 - Botucatu, SP Brasil, Tel.: (55 14) 3811 7262, Fax: (55 14) 3811 7206 - Botucatu - SP - Brazil
E-mail: summa.phyto@gmail.com