This work aimed the establishment of the best growth conditions of M. musicola mycelia in vitro through the analysis of the influence of different culture media (BDA, BDA/IFB, V8, V8/IFB, V8/CaCO3 and V8/CaCO3/IFB), combinations of carbon (dextrose, maltose, sucrose and xylose) and nitrogen (peptone, glycine, potassium nitrate and sodium nitrate) sources, medium pH values (6.8, 6.4, 5.7 and 4.9) and photoperiods (continuous darkness, alternating darkness/lightness and continuous lightness). At the end of the evaluation period, cultural parameters and the dry weights of colonies were annotated. BDA/IFB, V8/IFB and BDA culture media promoted the best mycelial growth. The experiments also demonstrated that dextrose and sucrose when combined with peptose as a nitrogen source, are the best carbon sources as they promoted the most vigorous mycelial growth. The use of BDA/IFB culture medium, with its final pH adjusted to 5.7, and a photoperiod of continuous darkness was the best condition for the growth of M. musicola.
Pseudocercospora musae; sigatoka disease; yellow sigatoka; banana
