Target |
Code |
Sequence (5’→3’) |
Amplicon Size (bp) |
Citation |
Universal (5S - 18S rDNA) |
194 |
TTAACTTGCCAGATCGGACG |
720 |
BLOK et al. (1997BLOK, V.C. et al. Comparison of sequences from the ribosomal DNA intergenic region of Meloidogyne mayaguensis and other major tropical root knot nematodes. Journal of Nematology, v.29, p.16-22, 1997. Available from: http://www.documentation.ird.fr/hor/fdi:010048922>. Accessed: Dec. 03, 2016. http://www.documentation.ird.fr/hor/fdi:...
) |
|
195 |
TCTAATGAGCCGTACGC |
|
|
Universal (D2/D3 28S rDNA) |
D2 |
ACAAGTACCGTGAGGGAAAGT |
500 |
NUNN (1992NUNN, G. Nematode molecular evolution. An investigation of evolutionary patterns among nematodes based upon DNA sequences. 1992. Ph.D. dissertation, University of Nottingham, UK, 1992. Available from: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334855>. Accessed: Mar. 15, 2017. http://ethos.bl.uk/OrderDetails.do?uin=u...
) |
|
D3 |
TGCGAAGGAACCAGCTACTA |
|
|
Universal (COII 16S mtRNA) |
C2F3 |
GGTCAATGTTCAGAAATTTGTGG |
- |
POWERS & HARRIS (1993POWERS, T.O.; HARRIS, T.S.A polymerase chain reaction method for identification of five major Meloidogyne species. Journal of Nematology , v.25, p.1-6, 1993. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2619349/>. Accessed: Jan. 14, 2017. https://www.ncbi.nlm.nih.gov/pmc/article...
) |
|
1108 |
TACCTTTGACCAATCACGCT |
|
|
M. arenaria (SCAR) |
Far |
TCGGCGATAGAGGTAAATGAC |
420 |
ZIJLSTRA (2000aZIJLSTRA, C. et al. Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterized amplified regions (SCAR) based PCR assays. Nematology , v.2, n.8, p.847-853, 2000a. Accessed: Dec. 03, 2017. doi: 10.1163/156854100750112798. https://doi.org/10.1163/1568541007501127...
) |
|
Rar |
TCGGCGATAGACACTACAAACT |
|
|
M. arenaria (SCAR) |
- |
TCGAGGGCATCTAATAAAGG |
950 |
DONG et al. (2001DONG, K. et al. Development of PCR primers to identify species of root-knot nematodes: Meloidogyne arenaria, M. hapla, M. incognita and M. javanica. Nematropica, v.31, n.2, p.271-280, 2001. Available from: http://journals.fcla.edu/nematropica/article/viewFile/69633/67293>. Accessed: Mar. 03, 2017. http://journals.fcla.edu/nematropica/art...
) |
|
- |
GGGCTGAATAATCAAAGGAA |
|
|
M. enterolobii (SCAR) |
- |
GAAATTGCTTTATTGTTACTAAG |
322 |
BLOK et al. (2002BLOK, V.C. et al. Mitochondrial differences distinguishing Meloidogyne mayaguensis from the major species of tropical root-knot nematodes. Nematology, v.4, n.7, p.773-781, 2002. Accessed: Mar. 09, 2017. doi: 10.1163/156854102760402559. https://doi.org/10.1163/1568541027604025...
) |
|
- |
TAGCCACAGCAAAATAGTTTTC |
|
|
M. enterolobii (SCAR) |
MK7-F |
GATCAGAGGCGGGCGCATTGCGA |
520 |
TIGANO et al. (2010TIGANO, M.S. et al. Genetic diversity of the root-knot nematode Meloidogyne enterolobii and development of a SCAR marker for this guava-damaging species. Plant Pathology , v.59, n.6, p.1054-1061, 2010. Available from: https://www.researchgate.net/publication/229721445_Genetic_diversity_of_the_root-knot_nematode_Meloidogyne_enterolobii_and_development_of_a_SCAR_marker_for_this_guava-damaging_species>. Accessed: Nov. 13, 2017. doi: 10.1111/j.1365-3059.2010.02350.x. https://www.researchgate.net/publication...
) |
|
MK7-R |
CGAACTCGCTCGAACTCGAC |
|
|
M. enterolobii (SCAR) |
Me-F |
AACTTTTGTGAAAGTGCCGCTG |
236 |
LONG et al. (2006LONG, H. et al. Development of a PCR diagnostic for the root-knot nematode Meloidogyne enterolobii. Acta Phytopathologica Sinica, v.36, p.109-115, 2006. Accessed: Mar. 05, 2017.) |
|
Me-R |
TCAGTTCAGGCAGGATCAACC |
|
|
M. ethiopica (SCAR) |
meth-F |
ATGCAGCCGCAGGGAACGTAGTTG |
350 |
CORREA et al. (2014CORREA, V.R. et al. Genetic diversity of the root-knot nematode Meloidogyne ethiopica and development of a species-specific SCAR marker for its diagnosis. Plant Pathology, v.63, n.2, p.476-483, 2014. Available from: http://onlinelibrary.wiley.com/doi/10.1111/ppa.12108/full>. Accessed: Jan. 17, 2017. doi: 10.1111/ppa.12108. http://onlinelibrary.wiley.com/doi/10.11...
) |
|
meth-R |
TGTTGTTTCATGTGCTTCGGCATC |
|
|
M. exígua (SCAR) |
exD15-F |
CATCCGTGCTGTAGCTGCGAG |
562 |
RANDIG et al. (2002RANDIG, O. et al. A species-specific satellite DNA family in the genome of the coffee root nematode Meloidogyne exigua: application to molecular diagnosis of the parasite. MolecularPlant Pathology , v.3, p.431-437, 2002. Available from: http://onlinelibrary.wiley.com/doi/10.1046/j.1364-3703.2002.00134.x/full>. Accessed: Dec. 12, 2016. doi: 10.1046/j.1364-3703.2002.00134.x http://onlinelibrary.wiley.com/doi/10.10...
) |
|
exD15-R |
CTCCGTGGGAAGAAAGACTG |
|
|
M. hapla (SCAR) |
- |
CAGGCCCTTCCAGCTAAAGA |
960 |
WILLIAMSON et al. (1997WILLIAMSON, V.M. et al. A PCR assay to identify and distinguish single juveniles of Meloidogyne hapla and M. chitwoodi. Journal of Nematology , v.29, n.1, p.9-15, 1997. Available from: http://journals.fcla.edu/jon/article/view/66873/64541>. Accessed: Dec. 03, 2016. http://journals.fcla.edu/jon/article/vie...
) |
|
- |
CTTCGTTGGGGAACTGAAGA |
|
|
M. hapla (SCAR) |
- |
TGACGGCGGTGAGTGCGA |
610 |
ZIJLSTRA (2000bZIJLSTRA, C. Identification of Meloidogyne chitwoodi, M. fallax and M. hapla based on SCAR-PCR: a powerful way of enabling reliable identification of populations or individuals that share common traits. European Journal ofPlant Pathology , v.106, n.3, p.283-290, 2000b. Available from: https://link.springer.com/content/pdf/10.1023%2FA%3A1008765303364.pdf>. Accessed: Dec. 03, 2017. doi: 10.1023/A:1008765303364. https://link.springer.com/content/pdf/10...
) |
|
- |
TGACGGCGGTACCTCATAG |
|
|
M. hapla (SCAR) |
- |
GGCTGAGCATAGTAGATGATGTT |
1500 |
DONG et al. (2001DONG, K. et al. Development of PCR primers to identify species of root-knot nematodes: Meloidogyne arenaria, M. hapla, M. incognita and M. javanica. Nematropica, v.31, n.2, p.271-280, 2001. Available from: http://journals.fcla.edu/nematropica/article/viewFile/69633/67293>. Accessed: Mar. 03, 2017. http://journals.fcla.edu/nematropica/art...
) |
|
- |
ACCCATTAAAGAGGAGTTTTGC |
|
|
M. incognita (SCAR) |
Finc |
CTCTGCCCAATGAGCTGTCC |
1200 |
ZIJLSTRA (2000aZIJLSTRA, C. et al. Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterized amplified regions (SCAR) based PCR assays. Nematology , v.2, n.8, p.847-853, 2000a. Accessed: Dec. 03, 2017. doi: 10.1163/156854100750112798. https://doi.org/10.1163/1568541007501127...
) |
|
Rinc |
CTCTGCCCTCACATTAAG |
|
|
M. incognita (SCAR) |
- |
TAGGCAGTAGGTTGTCGGG |
1350 |
DONG et al. (2001DONG, K. et al. Development of PCR primers to identify species of root-knot nematodes: Meloidogyne arenaria, M. hapla, M. incognita and M. javanica. Nematropica, v.31, n.2, p.271-280, 2001. Available from: http://journals.fcla.edu/nematropica/article/viewFile/69633/67293>. Accessed: Mar. 03, 2017. http://journals.fcla.edu/nematropica/art...
) |
|
- |
CAGATATCTCTGCATTGGTGC |
|
|
M. incognita (SCAR) |
Inc-K14-F |
GGGATGTGTAAATGCTCCTG |
399 |
RANDIG et al. (2002RANDIG, O. et al. A species-specific satellite DNA family in the genome of the coffee root nematode Meloidogyne exigua: application to molecular diagnosis of the parasite. MolecularPlant Pathology , v.3, p.431-437, 2002. Available from: http://onlinelibrary.wiley.com/doi/10.1046/j.1364-3703.2002.00134.x/full>. Accessed: Dec. 12, 2016. doi: 10.1046/j.1364-3703.2002.00134.x http://onlinelibrary.wiley.com/doi/10.10...
) |
|
Inc-K14-R |
CCCGCTACACCCTCAACTTC |
|
|
M. incognita (SCAR) |
MI-F |
GTGAGGATTCAGCTCCCCAG |
955 |
MENG et al. (2004MENG, Q.P. et al. PCR assays for rapid and sensitive identification of three major root-knot nematodes, Meloidogyne incognita, M. javanica and M. arenaria. Acta Phytopathologica Sinica , v.34, p.204-210, 2004. Accessed: Mar. 11, 2017.) |
|
MI-R |
ACGAGGAACATACTTCTCCGTCC |
|
|
M. incognita (SCAR) |
Mi2F4 |
ATGAAGCTAAGACTTTGGGCT |
300 |
KIEWNICK et al. (2013KIEWNICK, S. et al. Identification of the tropical root-knot nematode species Meloidogyne incognita, M. javanica and M. arenaria using a multiplex PCR assay. Nematology , v.15, p.891-894, 2013. Accessed: Feb. 17, 2017. doi: 10.1163/15685411-00002751. https://doi.org/10.1163/15685411-0000275...
) |
|
Mi1R1 |
TCCCGCTACACCCTCAACTTC |
|
|
M. javanica (SCAR) |
Fjav |
GGTGCGCGATTGAACTGAGC |
670 |
ZIJLSTRA (2000aZIJLSTRA, C. et al. Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterized amplified regions (SCAR) based PCR assays. Nematology , v.2, n.8, p.847-853, 2000a. Accessed: Dec. 03, 2017. doi: 10.1163/156854100750112798. https://doi.org/10.1163/1568541007501127...
) |
|
Rjav |
CAGGCCCTTCAGTGGAACTATAC |
|
|
M. javanica (SCAR) |
- |
CCTTAATGTCAACACTAGAGCC |
1650 |
DONG et al. (2001DONG, K. et al. Development of PCR primers to identify species of root-knot nematodes: Meloidogyne arenaria, M. hapla, M. incognita and M. javanica. Nematropica, v.31, n.2, p.271-280, 2001. Available from: http://journals.fcla.edu/nematropica/article/viewFile/69633/67293>. Accessed: Mar. 03, 2017. http://journals.fcla.edu/nematropica/art...
) |
|
- |
GGCCTTAACCGACAATTAGA |
|
|
M. javanica (SCAR) |
- |
ACGCTAGAATTCGACCCTGG |
517 |
MENG et al. (2004MENG, Q.P. et al. PCR assays for rapid and sensitive identification of three major root-knot nematodes, Meloidogyne incognita, M. javanica and M. arenaria. Acta Phytopathologica Sinica , v.34, p.204-210, 2004. Accessed: Mar. 11, 2017.) |
|
- |
GGTACCAGAAGCAGCCATGC |
|
|
M. paranaensis (SCAR) |
Par-F |
GCCCGACTCCATTTGACGGA |
208 |
RANDIG et al. (2002RANDIG, O. et al. A species-specific satellite DNA family in the genome of the coffee root nematode Meloidogyne exigua: application to molecular diagnosis of the parasite. MolecularPlant Pathology , v.3, p.431-437, 2002. Available from: http://onlinelibrary.wiley.com/doi/10.1046/j.1364-3703.2002.00134.x/full>. Accessed: Dec. 12, 2016. doi: 10.1046/j.1364-3703.2002.00134.x http://onlinelibrary.wiley.com/doi/10.10...
) |
|
Par-R |
CCGTCCAGATCCATCGAAGTC |
|
|
|
JMV1 |
GGATGGCGTGCTTTCAAC |
|
|
M. hapla, M. chitwoodie M. fallax (IGS - SCAR) |
JMV2 |
TTTCCCCTTATGATGTTTACCC |
440, 540, 670 |
WISHART et al. (2002WISHART, J. et al. Ribosomal intergenic spacer: a PCR diagnostic for Meloidogyne chitwoodi, M. fallax and M. hapla. Phytopathology , v.92, n.8, p.884-892, 2002. Available from: http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2002.92.8.884>. Accessed: Feb. 17, 2017. doi: 10.1094/PHYTO.2002.92.8.884. http://apsjournals.apsnet.org/doi/pdf/10...
) |
|
JMV |
AAAAATCCCCTCGAAAAATCCACC |
|
|