[Caracterização molecular dos genes Kappa-casein e β-lactoglobulina em gado negro anatoliano e raças Holstein]

Bayraktar, M.

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Animal Science and Technology and Inspection of Animal Products • Arq. Bras. Med. Vet. Zootec. 74 (01) • Jan-Feb 2022 • https://doi.org/10.1590/1678-4162-12497 copiar

[Caracterização molecular dos genes Kappa-casein e β-lactoglobulina em gado negro anatoliano e raças Holstein]

Autoria SCIMAGO INSTITUTIONS RANKINGS

RESUMO

κ-Cn e β-lactoglobulina são importantes genes candidatos associados à produção de leite e ao teor de proteína do leite. A presente investigação é realizada para determinar o status de polimorfismos dos genes κ-Cn e β-lactoglobulina nas raças Anatolian Black cattle e Holstein. A técnica PCR-RFLP foi utilizada para determinar os polimorfismos Kappa-Casein e β-lactoglobulina em ambas as raças de gado. A freqüência dos alelos do gado negro anatólio em termos de κ-Cn e β-lactoglobulina foi de 0,50 (A) 0,50 (B) e 0,20 (A) 0,80 (B) respectivamente, enquanto que em Holstein foi de 0,29 (A) 0,71 (B) e 0,44 (A) 0,56 (B) respectivamente. O teste do qui-quadrado mostrou que cada raça bovina estava em equilíbrio de Hardy-Weinberg (P>0,05).

Palavras-chave:
κ-Cn; β-lactoglobulina; Anatolian Black; Holstein; polimorfismo

Primer code	primer sequences	PCR bp	Sources	PCR conditions	RE
κ-Cn	F/5’-TGTGCTGAGTAGGTATCCTAGTTATGG-3’ R/5’GCGTTGTCTTCTTTGATGTCTCCTTAG-3’	453	*Barroso et al.,* (1998**BARROSO, A.; DUNNER, S.; CANON, J. Detection of bovine kappa-casein variants A, B, C, and E by means of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). J. Anim. Sci., v.76, p.1535-1538, 1998.)	95°C 5m, 95°C 10s, 62°C 20s, 72°C 30s, 35 cycles 72°C 5m	HinfI
β-lactoglobulin	F/5’-CGCTCCCCACCCCGTCCTCACC-3’ R/5’-CCCGTCCCCAGTCACCCACAGG-3’	434	*Zhao et al.,* (1999**ZHAO, C.J.; ZHANG, Y., LI, N. Research on the relationship between molecular genetic polymorphism of milk protein and dairy traits in Chinese Holsteins. J. Yellow Cattle Sci., v.25, p.13-15, 1999.)	94°C 3m, 94°C 30s, 60°C 40s, 72°C 1m, 39 cycles 72°C 10m	HaeIII

Cattle breed	N	Allele Frequency		Genotype frequency			χ²
Cattle breed	N	A	B	AA	AB	BB
Anatolian Black	100	0.50	0.50	0.30	0.40	0.30	2
Holstein	100	0.29	0.71	0.50	0.41	0.09	4.84
Allele and genotype frequencies for β-lactoglobulin gene
Anatolian Black	100	0.20	0.80	0.020	0.36	0.62	0.78
Holstein	100	0.44	0.56	0.26	0.36	0.38	3.63

	κ-CN gene
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	Crossbreds	0.87(A)	0.13(B)
	Busha	0.67(A)	0.33(B)
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β-lactoglobulin
References	Breed	Allele frequency
*Zhang et al.* (2006**ZHANG, R.; CHEN, H.; LEI, C. et al. Association between PCR-RFLP polymorphisms of five gene loci and milk traits in Chinese Holstein. Asian-australas. J. Anim. Sci., v.20, p.166-171, 2006.)	Chinese Holstein	0.27(A)	0.73(B)
Rachagani and Gupta (2008RACHAGANI, S.; GUPTA, I. D. Bovine kappa-casein gene polymorphism and its association with milk production traits. Genet. Mol. Biol., v.31, p.893-897, 2008.)	Sahiwal	0.17(A)	0.83(B)
	Tharparkar	0.39(A)	0.61(B)
*Karimi et al.* (2009**KARIMI, K.; BEIGI NASSIRI, M.T.; MIRZADEH, K. et al. Polymorphism of the b-lactoglobulin gene and its association with milk production traits in Iranian Najdi cattle. Iran. J. Biotechnol., v.7, p.82-85, 2009.)	Najdi cattle	0.09(A)	0.91(B)
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*Čítek et al.* (2019**ČÍTEK, J.; HANUSOVÁ, L.; LÍSKOVCOVÁ, L. et al. Polymorphisms in CSN3, CSN2 and LGB genes and their relation to milk production in dairy cattle in the Czech Republic. Acta Univ. Agric. Silvicult. Mendelianae Brunensis. v.67, p.19-24, 2019.)	Czech	0.47(A)	0.53(B)
*Ferreira et al.* (2019**FERREIRA, J.; GUILHERMINO, M.; LEITE, J. et al. Polymorphisms of leptin, β-lactoglobulin and pituitary transcription factor have no effect on milk characteristics in crossbred cows. Arq. Bras. Med. Vet. Zootec., v.71, p.715-719, 2019.)	Crossbred	0.40(A)	0.60(B)
*Pavlova et al.* (2019**PAVLOVA, N.; FILIPPOVA, N.; DODOKHOV, V. et al. The analysis of polymorphism of kappa-casein, β-lactoglobulin and prolactin genes among yakutian cattle and its influence on milk production. J. Agricult. Environ., v.2, p.1-8, 2019.)	Kholmogory	0.61(A)	0.39(B)
	Simmental Austrian selection	0.42(A)	0.58(B)
	Simmental local selection	0.29(A)	0.71(B)
*Barbosa et al.* (2019**BARBOSA, S.B.P.; ARAÚJO, Í.I.M.D.; MARTINS, M.F. et al. Genetic association of variations in the kappa-casein and β-lactoglobulin genes with milk traits in girolando cattle. Rev. Bras. Saúde Prod. Anim., v.20, p.1-12, 2019.)	Girolando	0.52(A)	0.48(B)

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E-mail: abmvz.artigo@gmail.com

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[1] Corresponding Author: mbayraktar@cu.edu.tr