Infection by microorganisms on soybean seeds obtained from plants treated with growth regulators

Castro, Paulo R.C.; Kimati, Hiroshi

doi:10.1590/S0071-12761981000100004

Abstracts

This research, deals with the effects of exogenous growth regulators on infection by microorganisms on soybean (Glycine max cv. Davis) seeds. To study the influence of the chemicals, soybean plants were sprayed with gibberellic acid (GA) 100 ppm, (2-chloroethyl) trimethylammonium chloride (CCC) 2,000 ppm, succinic acid-2,2-dimethy1hydrazide (SADH) 4,000 ppm, indolylacetic acid (IAA) 100 ppm, 2,3,5-triiodobenzoic acid (TIBA) 20 ppm (three applications), and Agrostemin (1g/10 ml/ 3 1). Application of growth regulators did not affect infect ion by microorganisms on soybean seeds. The prominent fungus isolated was Phomopsis sojae. Alternaria and Fusarium spp. were isolated from seeds. The presence of a bacterium on the seeds was observed. The delay in harvest and high humidity increased the number of seeds from which Phomopsis was recovered.

Para se verificar o efeito de fitoreguladores na incidência de microorganismos nas sementes de soja (Glycine max cv. Davis) efetuou-se a aplicação, em condições de campo, de ácido giberélico (GA) 100 ppm, cloreto (2-cloroetil) trimetilamônio (CCC) 2.000 ppm, ácido succínico-2,2-dimetílhidrazida -(SADH) 4.000 ppm, ácido indolilacético(IAA) 100 ppm, ácido 2,3,5- triiodobenzóico(TIBA) 20 ppm (3 vezes) e Agrostemin (1 g/10 ml/3 1), além do controle. Observou-se que aplicação de fitoreguladores nas plantas de soja não afetou a incidência de microorganismos nas sementes. Phomopsis sojae foi o principal fungo isolado das sementes. Verificou se ainda a ocorrência de Alternaria sp. e Fusarium sp. nas sementes de soja 'Davis'. Cercospora kikuchii também mostrou-se presente nas sementes de soja, além da ocorrência de bacteriose. Atraso na colheita e alta umidade aumentaram a incidência de Phanopsis.

Paulo R.C. Castro^I; Hiroshi Kimati^II

^IDepartamento de Botânica, E.S.A. ^"Luiz de Queiroz^", USP

^IIDepartamento de Fitopatologia, E.S.A. "Luiz de Queiroz", USP

RESUMO

Para se verificar o efeito de fitoreguladores na incidência de microorganismos nas sementes de soja (Glycine max cv. Davis) efetuou-se a aplicação, em condições de campo, de ácido giberélico (GA) 100 ppm, cloreto (2-cloroetil) trimetilamônio (CCC) 2.000 ppm, ácido succínico-2,2-dimetílhidrazida -(SADH) 4.000 ppm, ácido indolilacético(IAA) 100 ppm, ácido 2,3,5- triiodobenzóico(TIBA) 20 ppm (3 vezes) e Agrostemin (1 g/10 ml/3 1), além do controle. Observou-se que aplicação de fitoreguladores nas plantas de soja não afetou a incidência de microorganismos nas sementes. Phomopsis sojae foi o principal fungo isolado das sementes. Verificou se ainda a ocorrência de Alternaria sp. e Fusarium sp. nas sementes de soja 'Davis'. Cercospora kikuchii também mostrou-se presente nas sementes de soja, além da ocorrência de bacteriose. Atraso na colheita e alta umidade aumentaram a incidência de Phanopsis.

SUMMARY

This research, deals with the effects of exogenous growth regulators on infection by microorganisms on soybean (Glycine max cv. Davis) seeds. To study the influence of the chemicals, soybean plants were sprayed with gibberellic acid (GA) 100 ppm, (2-chloroethyl) trimethylammonium chloride (CCC) 2,000 ppm, succinic acid-2,2-dimethy1hydrazide (SADH) 4,000 ppm, indolylacetic acid (IAA) 100 ppm, 2,3,5-triiodobenzoic acid (TIBA) 20 ppm (three applications), and Agrostemin (1g/10 ml/ 3 1). Application of growth regulators did not affect infect ion by microorganisms on soybean seeds. The prominent fungus isolated was Phomopsis sojae. Alternaria and Fusarium spp. were isolated from seeds. The presence of a bacterium on the seeds was observed. The delay in harvest and high humidity increased the number of seeds from which Phomopsis was recovered.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

LITERATURA CITADA

Entregue para publicação em 27/01/1981

References

ATHOW, K.L.; LAVIOLETTE, F.A., 1973. Pod protection affects on soybean seed germination and infection with Diaporthe phaseolorum var. sojae and other microorganisms. Phytopath ology 63: 1021-1023.
ATUBRA, O.K.; KREZDORN, A.H., 1974. Responses of healthy and virus-infectsed citrus to growth regulator. Proc Fl. Sta. Hort. Soc. 86: 24-29.
BOCKMANN, H;., 1965. Lodging in wheat and its control. Mitt. Deut. Landwirt.-Ges. 80: 1095-1098.
BOLKAN, H.A.; SILVA, A.R.; CUPERTINO, F.P., 1976. Fungi associated with soybean and bean seeds and their control in Central Brazil. Plant Dis. Reptr. 60: 545-548.
BUCHENAUER, H., 1971· Einfluss einiger wuchshemmstoffe (CCC, CMH, AMO 1618, Phosphon D, B-9S5) auf die Fusarium and Ver tioillium - welke der tomate. Phytopathologische Zeitschrift 72: 53-66.
CASTRO, P.R.C.; MALAVOLTA, E., 1976. Ocorrência da podridão estilar em tomateiros (Lycopersieon esculentwn Mill.) sob efeito de reguladores de crescimento. Anais Esc. Sup. Agr. "Luiz de Queiroz" 33: 173-189.
CASTRO, P.R.C.; MALAVOLTA, E-, 1977. Influence og growth regulators upon mineral nutrition, osmotic potential, and incidence of blossom-end rot of tomato fruit.Turrialba 27:273-276.
CROSSAN, D.F.; FIELDHOUSE, D.J., 1964. A comparison of dwarfing and other compounds with and without fixed copper fungicide for control of bacterial spot of pepper. Plant Dis. Reptr. 48: 549-550.
DAFT, G.C.; LEBEN, C., 1972. Bacterial blight of soybeans, epidemiology of blicht outbreaks. Phytopathology 62: 57-62.
DHINGRA, O.D.; SILVA, J.F., 1978. Effect of weed control on the internally seedborn fungi in soybean seeds. Plant Dis. Reptr. 62: 513-516.
DIERCKS, R., 1965. The control of eye-spot disease of wheat (Cercosporella herpotrichoides) with chlorocholine chloride. Z. Pflanzenkrankh. Pflanzenshutz 72: 257-271.
ELLIS, M.A.; SINCLAIR, J.B., 1976. Effect of Benomyl field-sprays on internally-borne fungi, germination, and emergence of late-harvested soybean seeds. Phytopathology 66: 680 682.
FLIEGEL, P.; PARKER, K.G.; EDGERTON, L.J., 1966. Gibberellic acid treatment of sour cherry infected by sour cherry yellows virus: response to sprays applied throughout the growing season and the influence of environmental conditions. Plant Dis. Reptr. 50: 240-244.
HORN, N.L.; LEE, F.N.; CARVER, R.B., 1975. Effects of fungicides and pathogens on yields of soybeans. Plant Dis. Reptr. 59: 724-728.
JOSHI, R.D.; DUREY, L.N., 1975. Studies on the inhibition of cucumber mosaic virus in chilli (Capsicum annuum) III. Effect of growth regulators. Science and Culture 41: 351-353.
KARAS, J.G.; HAMNER, C.L.; DEZEEUW, D.J.; SELL, H.M., 1964. Inhibition of localized virus lesions by N-dimethylamino - succinamic acid. Nature 203: 1197.
KMETZ, K.; ELLETT, C.W.; SCHMITTHENNER, A.F., 1974. Isolation of seedborn Diaporte phaseolorun and Phomopsis from immature soybean seeds. Plant Dis. Repetr. 58: 978-982.
MOLOT, P.M.; NOURRISSEAU, J.G., 1974. Influence de quelques substances de croissance sur la sensibilité du fraiser aux attaques de Phytophthara cactorum. Fruits 29: 697-702.
OSWALD, T.H.; WYLLIE, T.D., 1973. Effects of growth regulator treatments on severity of charcoal rot disease of soybean. Plant Dis. Reptr. 57: 782-792.
PELSER, P.T., 1974. Influence of thiabendazole and benomyl on Alternaria rot in stored grapefruit. Citrus and Sub - Tropical Fruit Journal 486: 15"17.
RAWLINS, T.E., 1962. The inhibitory effect of 2-chloroethyl trimethylammonium chloride treatment on tobacco mosaic virus (TMV) multiplication. Plant Dis. Reptr. 46: 170.
ROSS, J.P., 1975. Effect of overhead irrigation and Benomyl sprays on late-season foliar diseases, seed infection, and yields of soybean. Plant Dis. Reptr. 59: 809-813.
RUSSEL, S., 1975. The role of cellulase produced by Verticillium albo-atrum in VerticiIlium wilt tomatoes. Phytopatho logiche Zeitschrift 82: 35-48.
SINGH, B.P.; MISRA, A.K., 1974. Effects of chemical in flowering of Chysccnthemum stunt virus-infected plants. Proc. Indian Acad. Sci. 80: 267-274.
SINHA, A.K.; WOOD, R.K.S., 1964. Control of Verticillium wilt of tomato plants with "Cycocel" (2-chloroethyl trimethylammonium chloride). Nature 202: 824.
TAHORI , A.S.; ZEIDLER, G.; HALEVY, A.H., 1965. Effect of some plant growth retarding compounds on three fungal diseases and one viral disease. Plant Dis. Reptr. 49: 775-777.
TENNE, F.D.; MENGISTU, A.; SINCLAIR, J.B., 1975. Occurrence and identification of Bacillus subtilis associated with soybean seeds from six geographical countries. Proc. Am. Phytopathol. Soc. 2: 91.
WILCOX, J.R.; LAVIOLETTE, F.A.; ATHOW, K.L., 1974. Deterioration of soybean seed quality associated with delayed harvest. Plant Dis. Reptr. 58: 130-133.

Infestação por microorganismos de sementes de soja obtidas de plantas tratadas com fitoreguladores

Infection by microorganisms on soybean seeds obtained from plants treated with growth regulators

Publication Dates

Publication in this collection
18 Apr 2012
Date of issue
1981

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] ATHOW, K.L.; LAVIOLETTE, F.A., 1973. Pod protection affects on soybean seed germination and infection with Diaporthe phaseolorum var. sojae and other microorganisms. Phytopath ology 63: 1021-1023.

[2] ATUBRA, O.K.; KREZDORN, A.H., 1974. Responses of healthy and virus-infectsed citrus to growth regulator. Proc Fl. Sta. Hort. Soc. 86: 24-29.

[3] BOCKMANN, H;., 1965. Lodging in wheat and its control. Mitt. Deut. Landwirt.-Ges. 80: 1095-1098.

[4] BOLKAN, H.A.; SILVA, A.R.; CUPERTINO, F.P., 1976. Fungi associated with soybean and bean seeds and their control in Central Brazil. Plant Dis. Reptr. 60: 545-548.

[5] BUCHENAUER, H., 1971· Einfluss einiger wuchshemmstoffe (CCC, CMH, AMO 1618, Phosphon D, B-9S5) auf die Fusarium and Ver tioillium - welke der tomate. Phytopathologische Zeitschrift 72: 53-66.

[6] CASTRO, P.R.C.; MALAVOLTA, E., 1976. Ocorrência da podridão estilar em tomateiros (Lycopersieon esculentwn Mill.) sob efeito de reguladores de crescimento. Anais Esc. Sup. Agr. "Luiz de Queiroz" 33: 173-189.

[7] CASTRO, P.R.C.; MALAVOLTA, E-, 1977. Influence og growth regulators upon mineral nutrition, osmotic potential, and incidence of blossom-end rot of tomato fruit.Turrialba 27:273-276.

[8] CROSSAN, D.F.; FIELDHOUSE, D.J., 1964. A comparison of dwarfing and other compounds with and without fixed copper fungicide for control of bacterial spot of pepper. Plant Dis. Reptr. 48: 549-550.

[9] DAFT, G.C.; LEBEN, C., 1972. Bacterial blight of soybeans, epidemiology of blicht outbreaks. Phytopathology 62: 57-62.

[10] DHINGRA, O.D.; SILVA, J.F., 1978. Effect of weed control on the internally seedborn fungi in soybean seeds. Plant Dis. Reptr. 62: 513-516.

[11] DIERCKS, R., 1965. The control of eye-spot disease of wheat (Cercosporella herpotrichoides) with chlorocholine chloride. Z. Pflanzenkrankh. Pflanzenshutz 72: 257-271.

[12] ELLIS, M.A.; SINCLAIR, J.B., 1976. Effect of Benomyl field-sprays on internally-borne fungi, germination, and emergence of late-harvested soybean seeds. Phytopathology 66: 680 682.

[13] FLIEGEL, P.; PARKER, K.G.; EDGERTON, L.J., 1966. Gibberellic acid treatment of sour cherry infected by sour cherry yellows virus: response to sprays applied throughout the growing season and the influence of environmental conditions. Plant Dis. Reptr. 50: 240-244.

[14] HORN, N.L.; LEE, F.N.; CARVER, R.B., 1975. Effects of fungicides and pathogens on yields of soybeans. Plant Dis. Reptr. 59: 724-728.

[15] JOSHI, R.D.; DUREY, L.N., 1975. Studies on the inhibition of cucumber mosaic virus in chilli (Capsicum annuum) III. Effect of growth regulators. Science and Culture 41: 351-353.

[16] KARAS, J.G.; HAMNER, C.L.; DEZEEUW, D.J.; SELL, H.M., 1964. Inhibition of localized virus lesions by N-dimethylamino - succinamic acid. Nature 203: 1197.

[17] KMETZ, K.; ELLETT, C.W.; SCHMITTHENNER, A.F., 1974. Isolation of seedborn Diaporte phaseolorun and Phomopsis from immature soybean seeds. Plant Dis. Repetr. 58: 978-982.

[18] MOLOT, P.M.; NOURRISSEAU, J.G., 1974. Influence de quelques substances de croissance sur la sensibilité du fraiser aux attaques de Phytophthara cactorum. Fruits 29: 697-702.

[19] OSWALD, T.H.; WYLLIE, T.D., 1973. Effects of growth regulator treatments on severity of charcoal rot disease of soybean. Plant Dis. Reptr. 57: 782-792.

[20] PELSER, P.T., 1974. Influence of thiabendazole and benomyl on Alternaria rot in stored grapefruit. Citrus and Sub - Tropical Fruit Journal 486: 15"17.

[21] RAWLINS, T.E., 1962. The inhibitory effect of 2-chloroethyl trimethylammonium chloride treatment on tobacco mosaic virus (TMV) multiplication. Plant Dis. Reptr. 46: 170.

[22] ROSS, J.P., 1975. Effect of overhead irrigation and Benomyl sprays on late-season foliar diseases, seed infection, and yields of soybean. Plant Dis. Reptr. 59: 809-813.

[23] RUSSEL, S., 1975. The role of cellulase produced by Verticillium albo-atrum in VerticiIlium wilt tomatoes. Phytopatho logiche Zeitschrift 82: 35-48.

[24] SINGH, B.P.; MISRA, A.K., 1974. Effects of chemical in flowering of Chysccnthemum stunt virus-infected plants. Proc. Indian Acad. Sci. 80: 267-274.

[25] SINHA, A.K.; WOOD, R.K.S., 1964. Control of Verticillium wilt of tomato plants with "Cycocel" (2-chloroethyl trimethylammonium chloride). Nature 202: 824.

[26] TAHORI , A.S.; ZEIDLER, G.; HALEVY, A.H., 1965. Effect of some plant growth retarding compounds on three fungal diseases and one viral disease. Plant Dis. Reptr. 49: 775-777.

[27] TENNE, F.D.; MENGISTU, A.; SINCLAIR, J.B., 1975. Occurrence and identification of Bacillus subtilis associated with soybean seeds from six geographical countries. Proc. Am. Phytopathol. Soc. 2: 91.

[28] WILCOX, J.R.; LAVIOLETTE, F.A.; ATHOW, K.L., 1974. Deterioration of soybean seed quality associated with delayed harvest. Plant Dis. Reptr. 58: 130-133.