Abstracts
The growth of Phaeodactylum tricornutum, cultured at 7,000 lux and 25º C, in twelve-day experiments using enriched water collected at the surface and 50.0 m depth from coastal waters offshore of Ubatuba area, was carried out. Different water enrichements were made by the aseptic addition of several nutrients, at each depth, according to Smayda (1964). The nitrogen out measured in terms of Carbon-14 assimilation and cloropyll concentration, was found to be a primary limiting factor for marine phytoplankton production.
Este trabalho teve como finalidade avaliar a qualidade das águas costeiras coletadas num ponto nas proximidades da Ilha Vitória (Lat. 23º45' S - Long. 45º01' W), na superfície e a 50,0 m de profundidade. Foram realizadas 176 ensaios biológicos com o auxílio de Phaeodactylum tricornutum, incubada em dez diferentes combinações de meios, além do controle. As respostas às diferentes condições ambientais fornecidas as culturas, foram obtidas baseando-se na diferenciação da biomassa em termos de clorofila-a, e na realização da fotossíntese em termos da assimilação do carbono-14. Os resultados demonstraram ser o nitrogênio o fator limitante primário para o crescimento do fitoplâncton.
Nutrient experiment using Phaeodactylum tricornutum as an assay organism
C. Teixeira; A. A. H. Vieira* * Fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo.
Instituto Oceanográfico da Universidade de São Paulo
SYNOPSIS
The growth of Phaeodactylum tricornutum, cultured at 7,000 lux and 25º C, in twelve-day experiments using enriched water collected at the surface and 50.0 m depth from coastal waters offshore of Ubatuba area, was carried out. Different water enrichements were made by the aseptic addition of several nutrients, at each depth, according to Smayda (1964). The nitrogen out measured in terms of Carbon-14 assimilation and cloropyll concentration, was found to be a primary limiting factor for marine phytoplankton production.
RESUMO
Este trabalho teve como finalidade avaliar a qualidade das águas costeiras coletadas num ponto nas proximidades da Ilha Vitória (Lat. 23º45' S - Long. 45º01' W), na superfície e a 50,0 m de profundidade.
Foram realizadas 176 ensaios biológicos com o auxílio de Phaeodactylum tricornutum, incubada em dez diferentes combinações de meios, além do controle.
As respostas às diferentes condições ambientais fornecidas as culturas, foram obtidas baseando-se na diferenciação da biomassa em termos de clorofila-a, e na realização da fotossíntese em termos da assimilação do carbono-14.
Os resultados demonstraram ser o nitrogênio o fator limitante primário para o crescimento do fitoplâncton.
Full text available only in PDF format.
Texto completo disponível apenas em PDF.
ACKNOWLEDGEMENTS
The authors whishes to express the best thanks to Dr. Falcão Paredes by the appreciation in preparing the manuscript, and to the "Fundação de Amparo À Pesquisa do Estado de São Paulo" for the grant awarded to the junior author.
REFERENCES
FOGG, G. E. 1958. Extracellular products of phytoplankton and the estimation of primary production. Rapp. P.-v. Réun. Cons. perm. int. Explor. Mer, 144:56-60.
GUILLARD, R. R. L. 1963. Organic sources of nitrogen for marine centric diatoms. Chap. 9, In: Openheiner, C. H., ed. - Symposium on marine microbiology. Springfield, I11., C. H. Thomas, p. 93-104.
MENZEL, D. W. & RYTHER, J. H. 1961. Annual variations in primary production of the Sargasso Sea, off Bermuda. Deep Sea Res., 7:282-288.
PARSONS, T. R. & STRICKLAND, J. D. H. 1963. Discussion of spectro-photometry determination of marine plant pigments, with revised equations for ascertaining chlorophyll and carotenoids. J. mar. Res., 21:155-163.
RICHARDS, F. A. & THOMPSON, T. G. 1952. The estimation and characterization of plankton population by pigment analysis. II. A spectrophotometry method for the estimation of plankton pigments. J. mar. Res., 11(2):156-172.
RYTHER, J. H. & GUILLARD, R. R. L. I960. Studies of marine planktonic diatoms. III. Detonula solitaria as an assay organism for vitamin B12. Woods Hole Oceanographic Institution, Appendix XI to Ref. 60-6:1-5.
SMAYDA, T. J. 1964. Enrichment experiments using the marine centric diatom Cyclotella nana (clone 13-1) as an assay organism. Oc. Publ. Grad. School Oceanogr. Univ. Rhode Island, (2):25-32.
TEIXEIRA, C. 1973. Preliminary studies of primary production in the Ubatuba region (Lat. 23º30' S - Long. 45º06' W), Brazil. Bolm Inst, oceanogr., S Paulo, 22:49-58.
(Recebido em 07/0utubro de 1974)
- FOGG, G. E. 1958. Extracellular products of phytoplankton and the estimation of primary production. Rapp. P.-v. Réun. Cons. perm. int. Explor. Mer, 144:56-60.
- GUILLARD, R. R. L. 1963. Organic sources of nitrogen for marine centric diatoms. Chap. 9, In: Openheiner, C. H., ed. - Symposium on marine microbiology. Springfield, I11., C. H. Thomas, p. 93-104.
- MENZEL, D. W. & RYTHER, J. H. 1961. Annual variations in primary production of the Sargasso Sea, off Bermuda. Deep Sea Res., 7:282-288.
- PARSONS, T. R. & STRICKLAND, J. D. H. 1963. Discussion of spectro-photometry determination of marine plant pigments, with revised equations for ascertaining chlorophyll and carotenoids. J. mar. Res., 21:155-163.
- RICHARDS, F. A. & THOMPSON, T. G. 1952. The estimation and characterization of plankton population by pigment analysis. II. A spectrophotometry method for the estimation of plankton pigments. J. mar. Res., 11(2):156-172.
- SMAYDA, T. J. 1964. Enrichment experiments using the marine centric diatom Cyclotella nana (clone 13-1) as an assay organism. Oc. Publ. Grad. School Oceanogr. Univ. Rhode Island, (2):25-32.
- TEIXEIRA, C. 1973. Preliminary studies of primary production in the Ubatuba region (Lat. 23ş30' S - Long. 45ş06' W), Brazil. Bolm Inst, oceanogr., S Paulo, 22:49-58.
Publication Dates
-
Publication in this collection
11 June 2012 -
Date of issue
1976
History
-
Received
07 Oct 1974
