Natural triploidy in Leporinus cf. elongatus bearing sex chromosomes

ANIMAL GENETICS

SHORT COMMUNICATION

Natural triploidy in Leporinus cf. elongatus bearing sex chromosomes

Wagner Franco Molina^I; Vladimir Pavan Margarido^II; Pedro Manoel Galetti Jr^III

^IDepartamento de Biologia Celular e Genética, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil

^IICentro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil

^IIIDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil

^{Send correspondence to}

ABSTRACT

Although several cases of natural triploidy in fish have already been described, spontaneous polyploidy in species with differentiated sex chromosomes are rare. We report the occurrence of a triploid fish (3n = 81) Leporinus cf. elongatus, a species characterized by a highly differentiated ZZ/ZW sex chromosome system, from the São Francisco river. The occurrence of a ZZZ triploid adult indicates the viability of this chromosome constitution in this fish.

Key words: polyploidy, triploid fish, ZW chromosomes, sex chromosomes, Anostomidae.

The family Anostomidae is composed of 12 genera, constituting a group relatively rich in species, distributed from South to Central America (Garavello, 1979). Eight species from this group, all from the genus Leporinus, exhibit a ZZ/ZW sex chromosome system with marked structural modifications between the sex chromosomes (Galetti et al., 1995; Venere et al., 2004; Molina and Galetti, 2006). The ZW chromosomes of these species point a monophyletic origin, apparently differentiated through processes of heterochromatinization (Galetti and Foresti, 1986; 1987). Leporinus is one of the more complexes groups among Characiformes and the largest in number of species, with 87 already described. Several attempts to subdivide it into additional genera and subgenera have failed (Garavello and Britski, 2003).

A diploid number of 2n = 54 is common among Leporinus species (Galetti et al., 1995) and no chromosome number variation has been reported in this genus thus far, despite of several reports of natural triploidy in different neotropical fish families (Characidae, Morelli et al., 1983; Fauaz et al., 1994; Centofante et al. 2001; Sternopygidae, Almeida-Toledo et al., 1985; Curimatidae, Venere and Galetti, 1985; Erythrinidae, Giuliano-Caetano and Bertollo, 1990; Thricomycteridae, Borin et al., 2002).

This study reports a case of spontaneous natural triploidy in Leporinus cf. elongatus with a highly differentiated ZZ/ZW sex chromosome system.

A total of 28 specimens of Leporinus cf. elongatus (12 males and 16 females) collected in the São Francisco river (Três Marias municipality; Brazil) were cytogenetically analyzed through the air-drying technique (Bertollo et al., 1978; Gold et al., 1990). the chromosome preparations were submitted to Ag-NOR (Howell and Black, 1980) and mithramycin staining (Schweizer, 1980).

Our results confirmed the previously reported 2n = 54 with a highly differentiated ZZ/ZW sex chromosome system, characterized by a large subtelocentric W chromosome and a submetacentric Z (Molina et al., 1998).

A young triploid male with 2n = 3x = 81 and presenting three copies of the Z chromosome was detected (Figure 1a, b). A previously reported conspicuous mithramycin positive heterochromatic block on the long arm of the Z chromosomes (Molina et al., 1998) was evident in the three Z chromosomes of the triploid complement (Figure 1c). No morphological differences were observed between the triploid and the diploid specimens.

The nucleolus organizer regions (NORs) were located near the telomere of the long arm of a large submetacentric chromosome in the diploid individuals (Figure 1d). Although good quality metaphases were not obtained for Ag-staining in the triploid specimen, while the interphase nuclei of diploid individuals has showed a maximum of two Ag-stained nucleoli in the triploid three nucleoli were detected (Figure 1e), indicating that these three constitutive NOR sites can be active in the triploid. Similar results were also observed in Trichomycterus davisi (Borin et al., 2002).

Polyploidy is believed to be an important mechanism in the chromosome evolution in some fish groups, such as Catostomidae and Salmonidae (reviewed in Comber and Smith, 2004) or in the neotropical Siluriformes, as Corydoras, Aspidoras and Brochis (Oliveira et al., 1993). Therefore, the polyploidy is particularly propitious to the evolutionary failure in species with differentiated sex chromosomes, likely due to a gene imbalance (Müller, 1925; Svartman et al., 2005). On the other hand, random natural triploidy appears more frequent in fish and it is mostly correlated to exposition to drastic temperature changes or other environmental conditions (Venere and Galetti, 1985), once the fecundation most of the time is external. However, natural triploidy in fish species with differentiated sex chromosomes is very rare.

In a set of 27 individuals of Characidium gomesi, which presents a ZZ/ZW system, Centofante et al. (2001) reported the occurrence of a triploid female. This triploid exhibited one W and two Z chromosomes, suggesting that it resulted from the fertilization of a normal (haploid) ovule by a non-reduced spermatozoid (2n). The presence of three Z chromosomes in the triploid male herein studied prevented us from determining if the triploidy was originated by maternal or paternal non-segregation.

The occurrence of triploids in cytogenetic analyses with samples relatively small as in L. cf. elongatus from the São Francisco river suggests that polyploidy events should happen in a larger frequency than could be imagined. However, one of the limitations to the evolution through the polyploidy in vertebrates has been attributed to the presence of differentiated sex chromosomes (Svartman et al., 2005).

The presence of sex chromosomes does not seem to impair the triploid development in L. cf. elongatus and our findings may have direct implications on the genetic manipulation of this fish and of other species bearing differentiated sex chromosomes.

Acknowledgements

The authors thank Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support.

Received: May 11, 2006; Accepted: November 23, 2006.

Associate Editor: Fausto Foresti

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Publication Dates

• Publication in this collection
  30 Aug 2007
• Date of issue
  2007

History

• Accepted
  23 Nov 2006
• Received
  11 May 2006