The article: “Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere induction”, published at Journal of Applied Oral Science 31(e-20230209):1-16. doi: 10.1590/1678-7757-2023-0209 was published with the following errors:
Where it reads, page 7
Figure 2- Characterization of hSCAPs. (a) The isolated cells can grow on plastic adherent culture vessels and reveal the typical fibroblastlike shape morphology. (b-c) The neural crest stem cells' derivative origin was demonstrated with β-III tubulin and nestin staining, respectively. (d) The number of isolated cells that expressed these markers (CD34−, CD73+, CD90+, CD105+, and CD146) are highly expressed. (e) The isolated cells can form colonies. (f-h) Multipotential differentiation abilities were demonstrated by adipogenesis, osteogenesis, and neurogenesis, respectively. Scale bars: a, f, g, and h = 100 μm, b and c = 50 μm, and e = 5 mm
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Figure 2- Characterization of hSCAPs. (A) The isolated cells can grow on plastic adherent culture vessels and reveal the typical fibroblastlike shape morphology. (B-C) The neural crest stem cells' derivative origin was demonstrated with β-III tubulin and nestin staining, respectively. (G) The number of isolated cells that expressed these markers (CD34−, CD73+, CD90+, CD105+, and CD146+) are highly expressed. (H) The isolated cells can form colonies. (D-F) Multipotential differentiation abilities were demonstrated by adipogenesis, osteogenesis, and neurogenesis, respectively. Scale bars: A, D, E, and F = 100 μm, B and C = 50 μm, and H = 5 mm
Where it reads, page 9
Figure 5- Immunofluorescence phenotyping of NSCs. (a-c) Single immunofluorescences profiling including DAPI, nestin, and SOX2, respectively. (d-f ) Double immunofluorescences profiling. (g) The intra-neurospheral cells were co-positively expressed and localized nuclei markers which were characterized as NSCs. Scale bars: a-g = 100 μm
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Figure 5- Immunofluorescence phenotyping of NSCs. (A-C and A’-C’) Single immunofluorescences profiling including DAPI, nestin, and SOX2, respectively. (D-F and D’-F’) Double immunofluorescences profiling. (G’) The intra-neurospheral cells were co-positively expressed and localized nuclei markers which were characterized as NSCs (H) The fluorescent intensity. Data were expressed as the mean±SD; n=3, ***p<0.001. Scale bars: A-G and A’-G’ = 100 μm
Where it reads, page 10
Figure 7- Gene profiling. (a-c) The neurospheres presented the increasing expression of NSCs profiling (<i>NES, SOX1, </i>and <i>PAX6</i>) when compared to the hSCAPs. Data were expressed as the mean±SD; n=3, **<i>p </i><0.01, ***<i>p</i> <0.001
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Figure 7- Gene profiling. (A-C) The neurospheres presented the increasing expression of NSCs profiling (NES, SOX1, and PAX6) when compared to the hSCAPs. Data were expressed as the mean±SD; n=3, **p<0.01, ***p<0.001
Where it reads, page 11
Figure 8- Functionality test. (a) The hSCAPs weakly expressed fluorescent calcium ions signal. (b) The intra-neurospheral cells obviously revealed calcium ions signal. (c) The hSCAPs presented a low and narrow dynamic change of calcium ions intensity (red, orange, and yellow lines). Importantly, higher and wider dynamic changes of calcium ions intensity were observed at intra-neurospheral cells (pink, dark blue, and light blue lines). Data were expressed as the mean intensity of calcium ions; n=3
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Figure 8- Functionality test. (A-D) The hSCAPs weakly expressed fluorescent calcium ions signal. (A’-D’) The intra-neurospheral cells obviously revealed calcium ions signal. (A’’-D’’) The hSCAPs presented a low and narrow dynamic change of calcium ions intensity (red, orange, and yellow lines). Importantly, higher and wider dynamic changes of calcium ions intensity were observed at intra-neurospheral cells (pink, dark blue, and light blue lines). Data were expressed as the mean intensity of calcium ions; n=3, Scale bar: A-D and A’-D’ = 100 μm
Publication Dates
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Publication in this collection
15 Dec 2023 -
Date of issue
2023