Evaluacion de un conjugado antirrabico por titulacion en diferentes microscopios fluorescentes

Larghi, O. P.; Oliva-Pinheiro, O.; Gonzalez-Luarca, E.

doi:10.1590/S0036-46651986000100001

Resúmenes

En cinco laboratorios se tituló una partida de conjugado antirrábico para inmunofluoresCencia con microscopios de tres marcas distintas, equipados con diferentes accesorios. Los títulos obtenidos variaron entre < 1:4 y 1:64, dependiendo de los accesorios utilizados. Estos resultados ponen de relieve la necesidad de titular los conjugados en cada laboratorio. Se mencionam los inconvenientes que pueden ocasionar la distribución del reactivo diluido de acuerdo con el título obtenido por el productor, o mezclado con suspensiones de cerebro de ratones normales e infectados con virus rábico.

Raiva; Imunofluorescência; Sorologia; Teste de qualidade diagnóstica

A batch of antirabies fluorescence conjugate was titrated in five laboratories, with three different makers of microscopes, equipped with a variety of accesories. The titers ranged between < 1:4 and 1:64, depending on the accesories used. The results indicate the need for each laboratory to titrate the conjugate. Also, the disadvantages of distributing the reagent diluted according to the titer obtained by the manufacturer, or mixed with the rabies-infected and normal mouse brain suspensions, are discussed.

ARTIGOS ORIGINAIS

Evaluacion de un conjugado antirrabico por titulacion en diferentes microscopios fluorescentes

Evaluation of a rabies conjugate by titration in different fluorescent microscopes

O. P. Larghi^I; O. Oliva-Pinheiro^II; E. Gonzalez-Luarca^III

^IOPS/OMS, Centro Panamericano de Zoonosis, Buenos Aires, Argentina

^IIFundação Oswaldo Cruz, Rio de Janeiro, Brasil

^IIIOMS/Organización Panamericana de la Salud, Brasília, Brasil

^{Dirección Actual}

RESUMEN

En cinco laboratorios se tituló una partida de conjugado antirrábico para inmunofluoresCencia con microscopios de tres marcas distintas, equipados con diferentes accesorios. Los títulos obtenidos variaron entre < 1:4 y 1:64, dependiendo de los accesorios utilizados. Estos resultados ponen de relieve la necesidad de titular los conjugados en cada laboratorio. Se mencionam los inconvenientes que pueden ocasionar la distribución del reactivo diluido de acuerdo con el título obtenido por el productor, o mezclado con suspensiones de cerebro de ratones normales e infectados con virus rábico.

Unitermos: Raiva Imunofluorescência Sorologia Teste de qualidade diagnóstica.

SUMMARY

A batch of antirabies fluorescence conjugate was titrated in five laboratories, with three different makers of microscopes, equipped with a variety of accesories. The titers ranged between < 1:4 and 1:64, depending on the accesories used. The results indicate the need for each laboratory to titrate the conjugate.

Also, the disadvantages of distributing the reagent diluted according to the titer obtained by the manufacturer, or mixed with the rabies-infected and normal mouse brain suspensions, are discussed.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Dirección Actual:

O. P. Larghi

OMS/Organización Panamericana de la Salud

Casilla 2117, Lima, Perú

Recebido para publicação em 20-12-1984.

1. CARSKI, T. R.; WILSNACK, R. E. & SIKES E. K. Pathogenesis of rabies in wildlife. II. Fluorescent antibody studies. Amer. J. Vet. Res. 23: 1048-1052, 1962.
2. CENTER FOR DISEASE CONTROL Rabies Surveillance Report. April-June 1974, p. 2, 1975.
3. CHERRY, W. B. Fluorescence emission with special reference to standardization in immunofluorescence. En: HOLLOW, E. J. (ed.) Standardization of immunofluorescence. Oxford, Blackwell Scientific Publications, p. 127-135, 1970.
4. FELTKAMP. Y. E. w. Titration of conjugates. En: HOLLOW, E. J. (ed.) Standardization of immunofluorescence. Oxford, Blackwell Scientific Publications, p. 189-192, 1970.
5. GOLDMAN, M. Fluorescent Antibody Methods. New York, Academic Press, 1968.
6. KISSLING, R. E. The fluorescent antibody test in rabies. En: BAER, G. M. (ed.) The natural history of rabies. New York, Academic Press., p. 401-416, 1975.
7. LARGHI, O. P. Prueba de anticuerpos fluorescentes para rabia. Centro Panamericano de Zoonosis OP5/OMS (Nota Técnica 8). Ramos Mejja, Argentina, 1975.
8. LARGHI, O. P.; GOYCOLEA,. N. E.; CASADEI, C. J & ZUCCÓN, R. J. E. Comparación de la diagnosis clinica de la rabia con los métodos de laboratorio. Rev. Pasteur (Bs. As.) 4: 5-8, 1971.
9. LARGHI, O. P. & JIMÉNEZ CH., E. Methods for accelerating the fluorescent antibody test for rabies diagnosis. Appl. Microbiol. 21: 611-613, 1971.
10. LENNETTE, E. H. & SCHMIDT, N. Diagnostic procedures for viral, rickettsial and chlamydial infection. 5th ed. Washington, D. C., American. Public Health Association. 1979.
11. LENNETTE, E. H.; WOODIE, J. D.¡ NAKAMURA, K. & MAGOFFIN, R. L. The diagnosis of rabies by fluorescent antibody method (FRA) emploiyng immune hamster serum. Hlth. Lab. Sci. 2: 24-34, 1965.
12. MCQUEEN, J. L.; LEWIS A. L. & SCHNEIDER, N. J. Rabies diagnosis by fluorescent antibody, I. Its evaluation in a public health laboratory. Amer. J. Pub. Hlth. 50: 1743-1752, 1962.
13. NADEL, M. K. & CARSKI, T. R. Investigation into factors influencing staining intensities of rabies immunofluorescent conjugates. Hlth. Lab. Sci. 1: 60-64, 1964.
14. NAIR, B. C. Fluorescent protein tracing, 3rd ed. Baltimore, Williams and Wilt ins Company, 1969.
15. TOMKINSON, A. B. Ilumination I. En: HOLLOW, E. J. (ed). Standardization of immunofluorescence. Oxford, Blackwell Scientific Publications, 1970, p. 5-10.
16. TRIMARCHI, C. V. & DEBBIE, J. G. Standardization and quantitation of immunofluorescence in the rabies fluorescent antibody test. Appl. Microbiol. 24: 609-612, 1972.
17. TZIANOBOS, T.; HEBERT G. A. & WHITE, L. A. Preparation of rabies fluorescein isothiocyanate-labeled immune globulin from mouse hyperimmune ascetic fluids. J. Clin. Microbiol. 3: 609-612, 1976.
18. WACHENDORFEE, G.; FRIEDERICH H. & FROST, J. W. Untersu Chungen über die Eignun verschiedener Konjugate zur fluoreszenzserologischen Diagnostik der Tolwutt. Dtsch. Tierarzti. Wschr. 88: 171-174, 1981.
19. YAMAMOTO, A. & KAWAMURA, A. Assessment of conjugates. En: HOLLOW, E. J. (ed). Standardization of immunofluorescence. Oxford, Blackwell, Scientific Publications, 1970, p. 181-183.

Fechas de Publicación

Publicación en esta colección
16 Oct 2012
Fecha del número
Feb 1986

Histórico

Recibido
2019

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. CARSKI, T. R.; WILSNACK, R. E. & SIKES E. K. Pathogenesis of rabies in wildlife. II. Fluorescent antibody studies. Amer. J. Vet. Res. 23: 1048-1052, 1962.

[2] 2. CENTER FOR DISEASE CONTROL Rabies Surveillance Report. April-June 1974, p. 2, 1975.

[3] 3. CHERRY, W. B. Fluorescence emission with special reference to standardization in immunofluorescence. En: HOLLOW, E. J. (ed.) Standardization of immunofluorescence. Oxford, Blackwell Scientific Publications, p. 127-135, 1970.

[4] 4. FELTKAMP. Y. E. w. Titration of conjugates. En: HOLLOW, E. J. (ed.) Standardization of immunofluorescence. Oxford, Blackwell Scientific Publications, p. 189-192, 1970.

[5] 5. GOLDMAN, M. Fluorescent Antibody Methods. New York, Academic Press, 1968.

[6] 6. KISSLING, R. E. The fluorescent antibody test in rabies. En: BAER, G. M. (ed.) The natural history of rabies. New York, Academic Press., p. 401-416, 1975.

[7] 7. LARGHI, O. P. Prueba de anticuerpos fluorescentes para rabia. Centro Panamericano de Zoonosis OP5/OMS (Nota Técnica 8). Ramos Mejja, Argentina, 1975.

[8] 8. LARGHI, O. P.; GOYCOLEA,. N. E.; CASADEI, C. J & ZUCCÓN, R. J. E. Comparación de la diagnosis clinica de la rabia con los métodos de laboratorio. Rev. Pasteur (Bs. As.) 4: 5-8, 1971.

[9] 9. LARGHI, O. P. & JIMÉNEZ CH., E. Methods for accelerating the fluorescent antibody test for rabies diagnosis. Appl. Microbiol. 21: 611-613, 1971.

[10] 10. LENNETTE, E. H. & SCHMIDT, N. Diagnostic procedures for viral, rickettsial and chlamydial infection. 5th ed. Washington, D. C., American. Public Health Association. 1979.

[11] 11. LENNETTE, E. H.; WOODIE, J. D.¡ NAKAMURA, K. & MAGOFFIN, R. L. The diagnosis of rabies by fluorescent antibody method (FRA) emploiyng immune hamster serum. Hlth. Lab. Sci. 2: 24-34, 1965.

[12] 12. MCQUEEN, J. L.; LEWIS A. L. & SCHNEIDER, N. J. Rabies diagnosis by fluorescent antibody, I. Its evaluation in a public health laboratory. Amer. J. Pub. Hlth. 50: 1743-1752, 1962.

[13] 13. NADEL, M. K. & CARSKI, T. R. Investigation into factors influencing staining intensities of rabies immunofluorescent conjugates. Hlth. Lab. Sci. 1: 60-64, 1964.

[14] 14. NAIR, B. C. Fluorescent protein tracing, 3rd ed. Baltimore, Williams and Wilt ins Company, 1969.

[15] 15. TOMKINSON, A. B. Ilumination I. En: HOLLOW, E. J. (ed). Standardization of immunofluorescence. Oxford, Blackwell Scientific Publications, 1970, p. 5-10.

[16] 16. TRIMARCHI, C. V. & DEBBIE, J. G. Standardization and quantitation of immunofluorescence in the rabies fluorescent antibody test. Appl. Microbiol. 24: 609-612, 1972.

[17] 17. TZIANOBOS, T.; HEBERT G. A. & WHITE, L. A. Preparation of rabies fluorescein isothiocyanate-labeled immune globulin from mouse hyperimmune ascetic fluids. J. Clin. Microbiol. 3: 609-612, 1976.

[18] 18. WACHENDORFEE, G.; FRIEDERICH H. & FROST, J. W. Untersu Chungen über die Eignun verschiedener Konjugate zur fluoreszenzserologischen Diagnostik der Tolwutt. Dtsch. Tierarzti. Wschr. 88: 171-174, 1981.

[19] 19. YAMAMOTO, A. & KAWAMURA, A. Assessment of conjugates. En: HOLLOW, E. J. (ed). Standardization of immunofluorescence. Oxford, Blackwell, Scientific Publications, 1970, p. 181-183.